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7yny
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of baculovirus LEF-3 from Helicoverpa armigera nucleopolyhedrovirus== | |
| + | <StructureSection load='7yny' size='340' side='right'caption='[[7yny]], [[Resolution|resolution]] 3.51Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7yny]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera_nucleopolyhedrovirus Helicoverpa armigera nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YNY FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yny OCA], [https://pdbe.org/7yny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yny RCSB], [https://www.ebi.ac.uk/pdbsum/7yny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yny ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q91BW6_9ABAC Q91BW6_9ABAC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Single-stranded DNA-binding proteins (SSBs) interact with single-stranded DNA (ssDNA) to form filamentous structures with various degrees of cooperativity, as a result of intermolecular interactions between neighboring SSB subunits on ssDNA. However, it is still challenging to perform structural studies on SSB-ssDNA filaments at high resolution using the most studied SSB models, largely due to the intrinsic flexibility of these nucleoprotein complexes. In this study, HaLEF-3, an SSB protein from Helicoverpa armigera nucleopolyhedrovirus, was used for in vitro assembly of SSB-ssDNA filaments, which were structurally studied at atomic resolution using cryo-electron microscopy. Combined with the crystal structure of ssDNA-free HaLEF-3 octamers, our results revealed that the three-dimensional rearrangement of HaLEF-3 induced by an internal hinge-bending movement is essential for the formation of helical SSB-ssDNA complexes, while the contacting interface between adjacent HaLEF-3 subunits remains basically intact. We proposed a local cooperative SSB-ssDNA binding model, in which, triggered by exposure to oligonucleotides, HaLEF-3 molecules undergo ring-to-helix transition to initiate continuous SSB-SSB interactions along ssDNA. Unique structural features revealed by the assembly of HaLEF-3 on ssDNA suggest that HaLEF-3 may represent a new class of SSB. | ||
| - | + | Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA.,Yin J, Fu Y, Rao G, Li Z, Tian K, Chong T, Kuang K, Wang M, Hu Z, Cao S Nucleic Acids Res. 2022 Dec 9;50(22):13100-13113. doi: 10.1093/nar/gkac1142. PMID:36477586<ref>PMID:36477586</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7yny" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Helicoverpa armigera nucleopolyhedrovirus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cao S]] | ||
| + | [[Category: Li Z]] | ||
| + | [[Category: Yin J]] | ||
Revision as of 06:33, 25 January 2023
Crystal structure of baculovirus LEF-3 from Helicoverpa armigera nucleopolyhedrovirus
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