7t31
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7t31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_R20291 Clostridioides difficile R20291]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T31 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7t31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_R20291 Clostridioides difficile R20291]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T31 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t31 OCA], [https://pdbe.org/7t31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t31 RCSB], [https://www.ebi.ac.uk/pdbsum/7t31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t31 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t31 OCA], [https://pdbe.org/7t31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t31 RCSB], [https://www.ebi.ac.uk/pdbsum/7t31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t31 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/Q185H8_CLOD6 Q185H8_CLOD6] | [https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/Q185H8_CLOD6 Q185H8_CLOD6] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Clostridioides difficile is a Gram-positive bacillus, which is a frequent cause of gastrointestinal infections triggered by the depletion of the gut microbiome. Because of the frequent recurrence of these infections after antibiotic treatment, mechanisms of C. difficile persistence and recurrence, including biofilm formation, are of increasing interest. Previously, our group and others found that type IV pili, filamentous helical appendages polymerized from protein subunits, promoted microcolony and biofilm formation in C. difficile. In Gram-negative bacteria, the ability of type IV pili to mediate bacterial self-association has been explained through interactions between the pili of adjacent cells, but type IV pili from several Gram-negative species are also required for natural competence through DNA-uptake. Here, we report the ability of two C. difficile pilin subunits, PilJ and PilW, to bind to DNA in vitro, as well as the defects in biofilm formation in the pilJ and pilW gene-interruption mutants. Additionally, we have resolved the X-ray crystal structure of PilW, which we use to model possible structural mechanisms for the formation of C. difficile biofilm through interactions between type IV pili and the DNA of the extracellular matrix. Taken together, our results provide further insight into the relationship between type IV pilus function and biofilm formation in C. difficile and, more broadly, suggest that DNA-recognition by type IV pili and related structures may have functional importance beyond DNA-uptake for natural competence. | ||
| - | + | ==See Also== | |
| - | + | *[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
X-ray Structure of Clostridiodies difficile PilW
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