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1iq6

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[[Image:1iq6.gif|left|200px]]
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{{STRUCTURE_1iq6| PDB=1iq6 | SCENE= }}
{{STRUCTURE_1iq6| PDB=1iq6 | SCENE= }}
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'''(R)-HYDRATASE FROM A. CAVIAE INVOLVED IN PHA BIOSYNTHESIS'''
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===(R)-HYDRATASE FROM A. CAVIAE INVOLVED IN PHA BIOSYNTHESIS===
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==Overview==
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The (R)-specific enoyl coenzyme A hydratase ((R)-hydratase) from Aeromonas caviae catalyzes the addition of a water molecule to trans-2-enoyl coenzyme A (CoA), with a chain-length of 4-6 carbons, to produce the corresponding (R)-3-hydroxyacyl-CoA. It forms a dimer of identical subunits with a molecular weight of about 14,000 and is involved in polyhydroxyalkanoate (PHA) biosynthesis. The crystal structure of the enzyme has been determined at 1.5-A resolution. The structure of the monomer consists of a five-stranded antiparallel beta-sheet and a central alpha-helix, folded into a so-called "hot dog" fold, with an overhanging segment. This overhang contains the conserved residues including the hydratase 2 motif residues. In dimeric form, two beta-sheets are associated to form an extended 10-stranded beta-sheet, and the overhangs obscure the putative active sites at the subunit interface. The active site is located deep within the substrate-binding tunnel, where Asp(31) and His(36) form a catalytic dyad. These residues are catalytically important as confirmed by site-directed mutagenesis and are possibly responsible for the activation of a water molecule and the protonation of a substrate molecule, respectively. Residues such as Leu(65) and Val(130) are situated at the bottom of the substrate-binding tunnel, defining the preference of the enzyme for the chain length of the substrate. These results provide target residues for protein engineering, which will enhance the significance of this enzyme in the production of novel PHA polymers. In addition, this study provides the first structural information of the (R)-hydratase family and may facilitate further functional studies for members of the family.
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(as it appears on PubMed at http://www.pubmed.gov), where 12409309 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12409309}}
==About this Structure==
==About this Structure==
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[[Category: Polyhydroxyalkanoate]]
[[Category: Polyhydroxyalkanoate]]
[[Category: The hydratase 2 motif]]
[[Category: The hydratase 2 motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:16:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:47:46 2008''

Revision as of 10:47, 1 July 2008

Image:1iq6.png

Template:STRUCTURE 1iq6

(R)-HYDRATASE FROM A. CAVIAE INVOLVED IN PHA BIOSYNTHESIS

Template:ABSTRACT PUBMED 12409309

About this Structure

1IQ6 is a Single protein structure of sequence from Aeromonas punctata. Full crystallographic information is available from OCA.

Reference

Crystal structure of the (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis., Hisano T, Tsuge T, Fukui T, Iwata T, Miki K, Doi Y, J Biol Chem. 2003 Jan 3;278(1):617-24. Epub 2002 Oct 29. PMID:12409309

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