4fyj
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4fyj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYJ FirstGlance]. <br> | <table><tr><td colspan='2'>[[4fyj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYJ FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyj OCA], [https://pdbe.org/4fyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyj RCSB], [https://www.ebi.ac.uk/pdbsum/4fyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyj ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyj OCA], [https://pdbe.org/4fyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyj RCSB], [https://www.ebi.ac.uk/pdbsum/4fyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/PTH_PSEAE PTH_PSEAE] The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis (By similarity). | [https://www.uniprot.org/uniprot/PTH_PSEAE PTH_PSEAE] The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis (By similarity). | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The peptidyl-tRNA hydrolase enzyme from the pathogenic bacterium Pseudomonas aeruginosa (Pth; EC 3.1.1.29) has been cloned, expressed in Escherichia coli and crystallized for X-ray structural analysis. Suitable crystals were grown using the sitting-drop vapour-diffusion method after one week of incubation against a reservoir solution consisting of 20% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10%(v/v) isopropyl alcohol. The crystals were used to obtain the three-dimensional structure of the native protein at 1.77 A resolution. The structure was determined by molecular replacement of the crystallographic data processed in space group P6(1)22 with unit-cell parameters a = b = 63.62, c = 155.20 A, alpha = beta = 90, gamma = 120 degrees . The asymmetric unit of the crystallographic lattice was composed of a single copy of the enzyme molecule with a 43% solvent fraction, corresponding to a Matthews coefficient of 2.43 A(3) Da(-1). The crystallographic structure reported here will serve as the foundation for future structure-guided efforts towards the development of novel small-molecule inhibitors specific to bacterial Pths. | ||
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| - | Recombinant production, crystallization and X-ray crystallographic structure determination of the peptidyl-tRNA hydrolase of Pseudomonas aeruginosa.,Hughes RC, McFeeters H, Coates L, McFeeters RL Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1472-6., doi: 10.1107/S1744309112045770. Epub 2012 Nov 28. PMID:23192026<ref>PMID:23192026</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4fyj" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Peptidyl-tRNA hydrolase|Peptidyl-tRNA hydrolase]] | *[[Peptidyl-tRNA hydrolase|Peptidyl-tRNA hydrolase]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal Structure of P. aeruginosa peptidyl-tRNA hydrolase
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