7uin
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM Structure of an Group II Intron Retroelement== | |
| + | <StructureSection load='7uin' size='340' side='right'caption='[[7uin]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7uin]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_rectale Eubacterium rectale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UIN FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uin OCA], [https://pdbe.org/7uin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uin RCSB], [https://www.ebi.ac.uk/pdbsum/7uin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uin ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A173ZME3_9FIRM A0A173ZME3_9FIRM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Group II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam the genome, integrating by reversal of forward splicing. Here we show that retrotransposition is achieved by a tertiary complex between a structurally elaborate ribozyme, its protein mobility factor, and a structured DNA substrate. We solved cryo-electron microscopy structures of an intact group IIC intron-maturase retroelement that was poised for integration into a DNA stem-loop motif. By visualizing the RNP before and after DNA targeting, we show that it is primed for attack and fits perfectly with its DNA target. This study reveals design principles of a prototypical retroelement and reinforces the hypothesis that group II introns are ancient elements of genetic diversification. | ||
| - | + | Structures of a mobile intron retroelement poised to attack its structured DNA target.,Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM Science. 2022 Nov 11;378(6620):627-634. doi: 10.1126/science.abq2844. Epub 2022 , Nov 10. PMID:36356138<ref>PMID:36356138</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7uin" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chung K]] | ||
| + | [[Category: Xu L]] | ||
Revision as of 10:06, 24 November 2022
CryoEM Structure of an Group II Intron Retroelement
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