1ivr

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[[Image:1ivr.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1ivr| PDB=1ivr | SCENE= }}
{{STRUCTURE_1ivr| PDB=1ivr | SCENE= }}
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'''STRUCTURE OF ASPARTATE AMINOTRANSFERASE'''
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===STRUCTURE OF ASPARTATE AMINOTRANSFERASE===
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==Overview==
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The crystal structure of mitochondrial aspartate aminotransferase (mAAT) of chicken complexed with erythro-beta-hydroxyaspartate has been determined at 2.4 A resolution. Pregrown crystals of mAAT complexed with the inhibitor maleate (closed enzyme conformation, orthorhombic space group C222(1)) were soaked in solutions of erythro-beta-hydroxyaspartate. The ligand exchange was monitored by microspectrophotometry. The active site turned out to be predominantly occupied by the carbinolamine intermediate. The carbinolamine is a true intermediate of the catalytic cycle forming the last covalently bound enzyme:substrate complex before release of the keto acid product. Occupancies of approximately 80% for the carbinolamine and of approximately 20% for the quinonoid intermediate were obtained. Two hydrogen bonds were identified that are potentially relevant for the accumulation of the carbinolamine intermediate: one to the hydroxyl group of Tyr 70* and the other to the epsilon-NH2 group of Lys 258.
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(as it appears on PubMed at http://www.pubmed.gov), where 8952476 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8952476}}
==About this Structure==
==About this Structure==
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[[Category: Carbinolamine]]
[[Category: Carbinolamine]]
[[Category: Erythro-beta-hydroxyaspartate]]
[[Category: Erythro-beta-hydroxyaspartate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:05:05 2008''

Revision as of 11:05, 1 July 2008

Image:1ivr.png

Template:STRUCTURE 1ivr

STRUCTURE OF ASPARTATE AMINOTRANSFERASE

Template:ABSTRACT PUBMED 8952476

About this Structure

1IVR is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Aspartate aminotransferase complexed with erythro-beta-hydroxyaspartate: crystallographic and spectroscopic identification of the carbinolamine intermediate., von Stosch AG, Biochemistry. 1996 Dec 3;35(48):15260-8. PMID:8952476

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