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1iw7

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[[Image:1iw7.gif|left|200px]]
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[[Image:1iw7.png|left|200px]]
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==Overview==
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{{ABSTRACT_12000971}}
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In bacteria, the binding of a single protein, the initiation factor sigma, to a multi-subunit RNA polymerase core enzyme results in the formation of a holoenzyme, the active form of RNA polymerase essential for transcription initiation. Here we report the crystal structure of a bacterial RNA polymerase holoenzyme from Thermus thermophilus at 2.6 A resolution. In the structure, two amino-terminal domains of the sigma subunit form a V-shaped structure near the opening of the upstream DNA-binding channel of the active site cleft. The carboxy-terminal domain of sigma is near the outlet of the RNA-exit channel, about 57 A from the N-terminal domains. The extended linker domain forms a hairpin protruding into the active site cleft, then stretching through the RNA-exit channel to connect the N- and C-terminal domains. The holoenzyme structure provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation.
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==About this Structure==
==About this Structure==
1IW7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The following page contains interesting information on the relation of 1IW7 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IW7 OCA].
1IW7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The following page contains interesting information on the relation of 1IW7 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IW7 OCA].
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==Reference==
 
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Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution., Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S, Nature. 2002 Jun 13;417(6890):712-9. Epub 2002 May 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12000971 12000971]
 
[[Category: Catabolite Activator Protein]]
[[Category: Catabolite Activator Protein]]
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:29:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:33:54 2008''

Revision as of 14:33, 26 June 2008

Image:1iw7.png

Template:STRUCTURE 1iw7

Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution


Template:ABSTRACT 12000971

About this Structure

1IW7 is a Protein complex structure of sequences from Thermus thermophilus. The following page contains interesting information on the relation of 1IW7 with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:33:54 2008

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