7r6q
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7r6q]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_BY4741 Saccharomyces cerevisiae BY4741]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R6Q FirstGlance]. <br> | <table><tr><td colspan='2'>[[7r6q]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_BY4741 Saccharomyces cerevisiae BY4741]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R6Q FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r6q OCA], [https://pdbe.org/7r6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r6q RCSB], [https://www.ebi.ac.uk/pdbsum/7r6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r6q ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.98Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r6q OCA], [https://pdbe.org/7r6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r6q RCSB], [https://www.ebi.ac.uk/pdbsum/7r6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r6q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/HAS1_YEAST HAS1_YEAST] ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.<ref>PMID:15049817</ref> <ref>PMID:15242642</ref> <ref>PMID:15718299</ref> | [https://www.uniprot.org/uniprot/HAS1_YEAST HAS1_YEAST] ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.<ref>PMID:15049817</ref> <ref>PMID:15242642</ref> <ref>PMID:15718299</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-scale RNA remodeling steps during the assembly of ribonucleoproteins (RNPs). We describe cryo-EM structures of 60S ribosomal biogenesis intermediates that reveal how context-specific RNA unwinding by the DEAD-box ATPase Spb4 results in extensive, sequence-specific remodeling of rRNA secondary structure. Multiple cis and trans interactions stabilize Spb4 in a post-catalytic, high-energy intermediate that drives the organization of the three-way junction at the base of rRNA domain IV. This mechanism explains how limited strand separation by DEAD-box ATPases is leveraged to provide non-equilibrium directionality and ensure efficient and accurate RNP assembly. | ||
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| + | Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.,Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP Nat Struct Mol Biol. 2022 Dec;29(12):1228-1238. doi: 10.1038/s41594-022-00874-9. , Epub 2022 Dec 8. PMID:36482249<ref>PMID:36482249</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7r6q" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Helicase 3D structures|Helicase 3D structures]] | ||
| + | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
| + | *[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
State E2 nucleolar 60S ribosome biogenesis intermediate - Foot region model
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