This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1iy6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1iy6.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1iy6.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1iy6| PDB=1iy6 | SCENE= }}
{{STRUCTURE_1iy6| PDB=1iy6 | SCENE= }}
-
'''Solution structure of OMSVP3 variant, P14C/N39C'''
+
===Solution structure of OMSVP3 variant, P14C/N39C===
-
==Overview==
+
<!--
-
The ovomucoid third domain from silver pheasant (OMSVP3), a typical Kazal-type inhibitor, strongly inhibits different serine proteases of various specificities, i.e., chymotrypsin, Streptomyces griseus protease, subtilisin, and elastase. Structural studies have suggested that conformational flexibility in the reactive site loop of the free inhibitor may be related to broad specificity of the ovomucoid. On the basis of the structural homology between OMSVP3 and ascidian trypsin inhibitor (ATI), which has a cystine-stabilized alpha-helical (CSH) motif in the sequence, we prepared the disulfide variant of OMSVP3, introducing an engineered disulfide bond between positions 14 and 39 near the reactive site (Met18-Glu19) by site-directed mutagenesis. The disulfide variant P14C/N39C retained potent inhibitory activities toward alpha-chymotrypsin (CHT) and S. griseus proteases A and B (SGPA and SGPB), while this variant lost most of its inhibitory activity toward porcine pancreatic elastase (PPE). We determined the solution structure of P14C/N39C, as well as that of wild-type OMSVP3, by two-dimensional nuclear magnetic resonance (2D NMR) methods and compared their structures to elucidate the structural basis of the inhibitory specificity change. For the molecular core consisting of a central alpha-helix and a three-stranded antiparallel beta-sheet, essentially no structural difference was detected between the two (pairwise rmsd value = 0.41 A). In contrast to this, a significant difference was detected in the loop from Cys8 to Thr17, where in P14C/N39C it has drawn approximately 4 A nearer the central helix to form the engineered Cys14-Cys39 bond. Concomitantly, the Tyr11-Pro12 cis-peptide linkage, which is highly conserved in ovomucoid third domains, was isomerized to the trans configuration. Such structural change in the loop near the reactive site may possibly affect the inhibitory specificity of P14C/N39C for the corresponding proteases.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12614146}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12614146 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12614146}}
==About this Structure==
==About this Structure==
-
1IY6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lophura_nycthemera Lophura nycthemera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IY6 OCA].
+
1IY6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lophura_nycthemera Lophura nycthemera]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IY6 OCA].
==Reference==
==Reference==
Line 40: Line 44:
[[Category: Solution structure]]
[[Category: Solution structure]]
[[Category: Variant]]
[[Category: Variant]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:34:12 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:12:19 2008''

Revision as of 11:12, 1 July 2008

Image:1iy6.png

Template:STRUCTURE 1iy6

Solution structure of OMSVP3 variant, P14C/N39C

Template:ABSTRACT PUBMED 12614146

About this Structure

1IY6 is a Single protein structure of sequence from Lophura nycthemera. Full experimental information is available from OCA.

Reference

Inhibitory specificity change of the ovomucoid third domain of the silver pheasant upon introduction of an engineered Cys14-Cys39 bond., Hemmi H, Kumazaki T, Yamazaki T, Kojima S, Yoshida T, Kyogoku Y, Katsu M, Shinohara F, Yokosawa H, Miura K, Kobayashi Y, Biochemistry. 2003 Mar 11;42(9):2524-34. PMID:12614146

Page seeded by OCA on Tue Jul 1 14:12:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1iy6"
Personal tools