This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1iyb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1iyb.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1iyb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1iyb| PDB=1iyb | SCENE= }}
{{STRUCTURE_1iyb| PDB=1iyb | SCENE= }}
-
'''Crystal Structure of the Nicotiana glutinosa Ribonuclease NW'''
+
===Crystal Structure of the Nicotiana glutinosa Ribonuclease NW===
-
==Overview==
+
<!--
-
Ribonuclease NW (RNase NW), the wound-inducible RNase in Nicotiana glutinosa leaves, preferentially cleaves guanylic acid. We expressed the cDNA encoding RNase NW in the methylotrophic yeast Pichia pastoris using the expression vector pPIC9K, and the resulting recombinant RNase NW (ryRNaseNW) secreted into medium was purified to apparent homogeneity using column chromatography. The crystal structure of ryRNase NW bound to 5'-GMP was determined at 1.5 A resolution by molecular replacement with tomato RNase LE as a search model. The RNase NW structurally belongs to the (alpha + beta) class of proteins, having eight helices (five alpha-helices and three 3(10) helices) and six beta-strands, and its structure is highly similar to those of other plant RNases, including a uridylic acid preferential RNase MC1 from bitter gourd seeds. The guanine ring of 5'-GMP lies in a hydrophobic pocket of the molecular surface composed of Tyr17, Tyr71, Ala80, Leu79, and Phe89: the guanine base is sandwiched between aromatic side chains of Tyr17 and Phe89. In addition, the guanine base is firmly stabilized by a network of hydrogen bonds of the side chains of Gln12 and Thr78, as well as of the main chain of Leu79. Therefore, Gln12, Tyr17, Thr78, Leu79, and Phe89 are responsible for recognition of the guanine base by RNase NW, findings which provide insight into the manner in which RNase NW preferentially cleaves guanylic acid.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12484757}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12484757 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12484757}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:34:36 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:12:54 2008''

Revision as of 11:12, 1 July 2008

Image:1iyb.png

Template:STRUCTURE 1iyb

Crystal Structure of the Nicotiana glutinosa Ribonuclease NW

Template:ABSTRACT PUBMED 12484757

About this Structure

1IYB is a Single protein structure of sequence from Nicotiana glutinosa. Full crystallographic information is available from OCA.

Reference

Guanine binding site of the Nicotiana glutinosa ribonuclease NW revealed by X-ray crystallography., Kawano S, Kakuta Y, Kimura M, Biochemistry. 2002 Dec 24;41(51):15195-202. PMID:12484757

Page seeded by OCA on Tue Jul 1 14:12:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1iyb"
Personal tools