1iyi

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{{STRUCTURE_1iyi| PDB=1iyi | SCENE= }}
{{STRUCTURE_1iyi| PDB=1iyi | SCENE= }}
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'''CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE'''
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===CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE===
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==Overview==
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Here we report the crystal structures of human hematopoietic prostaglandin (PG) D synthase bound to glutathione (GSH) and Ca2+ or Mg2+. Using GSH as a cofactor, prostaglandin D synthase catalyzes the isomerization of PGH2 to PGD2, a mediator for allergy response. The enzyme is a homodimer, and Ca2+ or Mg2+ increases its activity to approximately 150% of the basal level, with half maximum effective concentrations of 400 microM for Ca2+ and 50 microM for Mg2+. In the Mg2+-bound form, the ion is octahedrally coordinated by six water molecules at the dimer interface. The water molecules are surrounded by pairs of Asp93, Asp96 and Asp97 from each subunit. Ca(2+) is coordinated by five water molecules and an Asp96 from one subunit. The Asp96 residue in the Ca2+-bound form makes hydrogen bonds with two guanidium nitrogen atoms of Arg14 in the GSH-binding pocket. Mg2+ alters the coordinating water structure and reduces one hydrogen bond between Asp96 and Arg14, thereby changing the interaction between Arg14 and GSH. This effect explains a four-fold reduction in the K(m) of the enzyme for GSH. The structure provides insights into how Ca2+ or Mg2+ binding activates human hematopoietic PGD synthase.
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(as it appears on PubMed at http://www.pubmed.gov), where 12627223 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12627223}}
==About this Structure==
==About this Structure==
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[[Category: Pgd]]
[[Category: Pgd]]
[[Category: Sigma-class gst]]
[[Category: Sigma-class gst]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:35:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:13:22 2008''

Revision as of 11:13, 1 July 2008

Image:1iyi.png

Template:STRUCTURE 1iyi

CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE

Template:ABSTRACT PUBMED 12627223

About this Structure

1IYI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Mechanism of metal activation of human hematopoietic prostaglandin D synthase., Inoue T, Irikura D, Okazaki N, Kinugasa S, Matsumura H, Uodome N, Yamamoto M, Kumasaka T, Miyano M, Kai Y, Urade Y, Nat Struct Biol. 2003 Apr;10(4):291-6. PMID:12627223

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