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1ize

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{{STRUCTURE_1ize| PDB=1ize | SCENE= }}
{{STRUCTURE_1ize| PDB=1ize | SCENE= }}
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'''Crystal structure of Aspergillus oryzae Aspartic proteinase complexed with pepstatin'''
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===Crystal structure of Aspergillus oryzae Aspartic proteinase complexed with pepstatin===
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==Overview==
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The X-ray structures of Aspergillus oryzae aspartic proteinase (AOAP) and its complex with inhibitor pepstatin have been determined at 1.9A resolution. AOAP was crystallized in an orthorhombic system with the space group P2(1)2(1)2(1) and cell dimensions of a=49.4A, b=79.4A, and c=93.6A. By the soaking of pepstatin, crystals are transformed into a monoclinic system with the space group C2 and cell dimensions of a=106.8A, b=38.6A, c=78.7A, and beta=120.3 degrees. The structures of AOAP and AOAP/pepstatin complex were refined to an R-factor of 0.177 (R(free)=0.213) and of 0.185 (0.221), respectively. AOAP has a crescent-shaped structure with two lobes (N-lobe and C-lobe) and the deep active site cleft is constructed between them. At the center of the active site cleft, two Asp residues (Asp33 and Asp214) form the active dyad with a hydrogen bonding solvent molecule between them. Pepstatin binds to the active site cleft via hydrogen bonds and hydrophobic interactions with the enzyme. The structures of AOAP and AOAP/pepstatin complex including interactions between the enzyme and pepstatin are very similar to those of other structure-solved aspartic proteinases and their complexes with pepstatin. Generally, aspartic proteinases cleave a peptide bond between hydrophobic amino acid residues, but AOAP can also recognize the Lys/Arg residue as well as hydrophobic amino acid residues, leading to the activation of trypsinogen and chymotrypsinogen. The X-ray structure of AOAP/pepstatin complex and preliminary modeling show two possible sites of recognition for the positively charged groups of Lys/Arg residues around the active site of AOAP.
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{{ABSTRACT_PUBMED_12595261}}
==About this Structure==
==About this Structure==
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[[Category: Acid protease]]
[[Category: Acid protease]]
[[Category: Sugar binding]]
[[Category: Sugar binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:37:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:15:59 2008''

Revision as of 11:16, 1 July 2008

Image:1ize.png

Template:STRUCTURE 1ize

Crystal structure of Aspergillus oryzae Aspartic proteinase complexed with pepstatin

Template:ABSTRACT PUBMED 12595261

About this Structure

1IZE is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Crystal structures of Aspergillus oryzae aspartic proteinase and its complex with an inhibitor pepstatin at 1.9A resolution., Kamitori S, Ohtaki A, Ino H, Takeuchi M, J Mol Biol. 2003 Mar 7;326(5):1503-11. PMID:12595261

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