User:Victoria Fisk/Canine Parvovirus

This is a default text for your page Victoria Fisk/Canine Parvovirus. Click above on edit this page to modify. Be careful with the < and > signs.

You may include any references to papers as in: the use of JSmol in Proteopedia ^[1] or to the article describing Jmol ^[2] to the rescue.

History

Function

CPV2 is a non-enveloped single-stranded DNA virus in the Parvoviridae family. The name comes from the Latin parvus, meaning small, as the virus is only 20 to 26 nm in diameter. It has an icosahedral symmetry. The genome is about 5000 nucleotides long.[25] CPV2 continues to evolve, and the success of new strains seems to depend on extending the range of hosts affected and improved binding to its receptor, the canine transferrin receptor.[26] CPV2 has a high rate of evolution, possibly due to a rate of nucleotide substitution that is more like RNA viruses such as Influenzavirus A.[27] In contrast, FPV seems to evolve only through random genetic drift.[28]

CPV2 affects dogs, wolves, foxes, and other canids. CPV2a and CPV2b have been isolated from a small percentage of symptomatic cats and is more common than feline panleukopenia in big cats.[29]

Previously it has been thought that the virus does not undergo cross species infection. However studies in Vietnam have shown that CPV2 can undergo minor antigenic shift and natural mutation to infect felids. Analyses of feline parvovirus (FPV) isolates in Vietnam and Taiwan revealed that more than 80% of the isolates were of the canine parvovirus type, rather than feline panleukopenia virus (FPLV).[30] CPV2 may spread to cats easier than dogs and undergo faster rates of mutation within that species.

Disease

Canine parvovirus (CPV) is a highly contagious viral disease of dogs that most often causes gastrointestinal distress in puppies. . A rare variant of the disease may be seen in very young (neonatal) puppies is myocarditis (an inflammation of the heart muscle).

Diagnosis

Diagnosis is made through detection of CPV2 in the feces by either an ELISA or a hemagglutination test, or by electron microscopy. PCR has become available to diagnose CPV2, and can be used later in the disease when potentially less virus is being shed in the feces that may not be detectable by ELISA.[4] Clinically, the intestinal form of the infection can sometimes be confused with coronavirus or other forms of enteritis. Parvovirus, however, is more serious and the presence of bloody diarrhea, a low white blood cell count, and necrosis of the intestinal lining also point more towards parvovirus, especially in an unvaccinated dog. The cardiac form is typically easier to diagnose because the symptoms are distinct.[5]

Treatment

Survival rate depends on how quickly CPV is diagnosed, the age of the dog, and how aggressive the treatment is. There is no approved treatment, and the current standard of care is supportive care, involving extensive hospitalization, due to severe dehydration and potential damage to the intestines and bone marrow. A CPV test should be given as early as possible if CPV is suspected in order to begin early treatment and increase survival rate if the disease is found.Supportive care ideally also consists of crystalloid IV fluids and/or colloids (e.g., Hetastarch), antinausea injections (antiemetics) such as maropitant, metoclopramide, dolasetron, ondansetron and prochlorperazine, and broad-spectrum antibiotic injections such as cefazolin/enrofloxacin, ampicillin/enrofloxacin, metronidazole, timentin, or enrofloxacin.[6] IV fluids are administered and antinausea and antibiotic injections are given subcutaneously, intramuscularly, or intravenously. The fluids are typically a mix of a sterile, balanced electrolyte solution, with an appropriate amount of B-complex vitamins, dextrose, and potassium chloride. Analgesic medications can be used to counteract the intestinal discomfort caused by frequent bouts of diarrhea; however, the use of opioid analgesics can result in secondary ileus and decreased motility.

Relevance

Structural highlights

CPV is an icosahedral capsid structure measuring 26 nm in diameter. It contains a major capsid protein, known as VP2. VP1 is a minor structural protein that is present with a specific N-terminal peptide inside the capsid. Each capsid subunit is formed from an eight-stranded scene name='93/933985/Assymetric_unit/1'>antiparallel β-barrel motif<</scene>. This motif is a common motif found in many viruses. The CPV surface is made up of loops that connect the β strands. Each icosahedral 2-fold axis contains "spikes" that recognize receptivity and antigenicity of the host.