7uwq
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7uwq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UWQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[7uwq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UWQ FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uwq OCA], [https://pdbe.org/7uwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uwq RCSB], [https://www.ebi.ac.uk/pdbsum/7uwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uwq ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.05Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uwq OCA], [https://pdbe.org/7uwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uwq RCSB], [https://www.ebi.ac.uk/pdbsum/7uwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uwq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/W9BAW3_KLEPN W9BAW3_KLEPN] Catalyzes the hydrolysis of the N-glycosidic bond of AMP to form adenine and ribose 5-phosphate. Involved in regulation of AMP concentrations.[HAMAP-Rule:MF_01932] | [https://www.uniprot.org/uniprot/W9BAW3_KLEPN W9BAW3_KLEPN] Catalyzes the hydrolysis of the N-glycosidic bond of AMP to form adenine and ribose 5-phosphate. Involved in regulation of AMP concentrations.[HAMAP-Rule:MF_01932] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Klebsiella pneumoniae is a bacterial pathogen that is increasingly responsible for hospital-acquired pneumonia and sepsis. Progressive development of antibiotic resistance has led to higher mortality rates and creates a need for novel treatments. Because of the essential role that nucleotides play in many bacterial processes, enzymes involved in purine and pyrimidine metabolism and transport are ideal targets for the development of novel antibiotics. Herein we describe the structure of K. pneumoniae adenosine monophosphate nucleosidase (KpAmn), a purine salvage enzyme unique to bacteria, as determined by cryoelectron microscopy. The data detail a well conserved fold with a hexameric overall structure and clear density for the putative active site residues. Comparison to the crystal structures of homologous prokaryotic proteins confirms the presence of many of the conserved structural features of this protein yet reveals differences in distal loops in the absence of crystal contacts. This first cryo-EM structure of an Amn enzyme provides a basis for future structure-guided drug development and extends the accuracy of structural characterization of this family of proteins beyond this clinically relevant organism. | ||
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| + | Structure of Klebsiella pneumoniae adenosine monophosphate nucleosidase.,Richardson BC, Shek R, Van Voorhis WC, French JB PLoS One. 2022 Oct 20;17(10):e0275023. doi: 10.1371/journal.pone.0275023. , eCollection 2022. PMID:36264993<ref>PMID:36264993</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7uwq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Klebsiella pneumoniae adenosine monophosphate nucleosidase
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