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1j12

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{{STRUCTURE_1j12| PDB=1j12 | SCENE= }}
{{STRUCTURE_1j12| PDB=1j12 | SCENE= }}
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'''Beta-Amylase from Bacillus cereus var. mycoides in Complex with alpha-EBG'''
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===Beta-Amylase from Bacillus cereus var. mycoides in Complex with alpha-EBG===
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==Overview==
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The crystal structures of beta-amylase from Bacillus cereus var. mycoides in complexes with five inhibitors were solved. The inhibitors used were three substrate analogs, i.e. glucose, maltose (product), and a synthesized compound, O-alpha-D-glucopyranosyl-(1--&gt;4)-O-alpha-D-glucopyranosyl-(1--&gt;4)-D-xylopy ranose (GGX), and two affinity-labeling reagents with an epoxy alkyl group at the reducing end of glucose. For all inhibitors, one molecule was bound at the active site cleft and the non-reducing end glucose of the four inhibitors except GGX was located at subsite 1, accompanied by a large conformational change of the flexible loop (residues 93-97), which covered the bound inhibitor. In addition, another molecule of maltose or GGX was bound about 30 A away from the active site. A large movement of residues 330 and 331 around subsite 3 was also observed upon the binding of GGX at subsites 3 to 5. Two affinity-labeling reagents, alpha-EPG and alpha-EBG, were covalently bound to a catalytic residue (Glu-172). A substrate recognition mechanism for the beta-amylase was discussed based on the modes of binding of these inhibitors in the active site cleft.
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(as it appears on PubMed at http://www.pubmed.gov), where 12761294 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12761294}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Raw-starch binding domain]]
[[Category: Raw-starch binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:40:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:20:16 2008''

Revision as of 11:20, 1 July 2008

Image:1j12.png

Template:STRUCTURE 1j12

Beta-Amylase from Bacillus cereus var. mycoides in Complex with alpha-EBG

Template:ABSTRACT PUBMED 12761294

About this Structure

1J12 is a Single protein structure of sequence from Bacillus cereus. Full crystallographic information is available from OCA.

Reference

Crystal structures of beta-amylase from Bacillus cereus var mycoides in complexes with substrate analogs and affinity-labeling reagents., Oyama T, Miyake H, Kusunoki M, Nitta Y, J Biochem. 2003 Apr;133(4):467-74. PMID:12761294

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