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4ipn
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ipn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPN FirstGlance]. <br> | <table><tr><td colspan='2'>[[4ipn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPN FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_900096:thio-alpha-cellobiose+6-phosphate'>PRD_900096</scene>, <scene name='pdbligand=RTG:6-O-phosphono-alpha-L-idopyranose'>RTG</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.411Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_900096:thio-alpha-cellobiose+6-phosphate'>PRD_900096</scene>, <scene name='pdbligand=RTG:6-O-phosphono-alpha-L-idopyranose'>RTG</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ipn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipn OCA], [https://pdbe.org/4ipn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ipn RCSB], [https://www.ebi.ac.uk/pdbsum/4ipn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipn ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ipn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipn OCA], [https://pdbe.org/4ipn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ipn RCSB], [https://www.ebi.ac.uk/pdbsum/4ipn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipn ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A0H2UP35_STRPN A0A0H2UP35_STRPN] | [https://www.uniprot.org/uniprot/A0A0H2UP35_STRPN A0A0H2UP35_STRPN] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The 6-phospho-beta-glucosidase BglA-2 (EC 3.2.1.86) from glycoside hydrolase family 1 (GH-1) catalyzes the hydrolysis of beta-1,4-linked cellobiose 6-phosphate (cellobiose-6'P) to yield glucose and glucose 6-phosphate. Both reaction products are further metabolized by the energy-generating glycolytic pathway. Here, we present the first crystal structures of the apo and complex forms of BglA-2 with thiocellobiose-6'P (a non-metabolizable analog of cellobiose-6'P) at 2.0 and 2.4 A resolution, respectively. Similar to other GH-1 enzymes, the overall structure of BglA-2 from Streptococcus pneumoniae adopts a typical (beta/alpha)8 TIM-barrel, with the active site located at the center of the convex surface of the beta-barrel. Structural analyses, in combination with enzymatic data obtained from site-directed mutant proteins, suggest that three aromatic residues, Tyr(126), Tyr(303), and Trp(338), at subsite +1 of BglA-2 determine substrate specificity with respect to 1,4-linked 6-phospho-beta-glucosides. Moreover, three additional residues, Ser(424), Lys(430), and Tyr(432) of BglA-2, were found to play important roles in the hydrolytic selectivity toward phosphorylated rather than non-phosphorylated compounds. Comparative structural analysis suggests that a tryptophan versus a methionine/alanine residue at subsite -1 may contribute to the catalytic and substrate selectivity with respect to structurally similar 6-phospho-beta-galactosidases and 6-phospho-beta-glucosidases assigned to the GH-1 family. | ||
| - | |||
| - | Structural Insights into the Substrate Specificity of a 6-Phospho-beta-glucosidase BglA-2 from Streptococcus pneumoniae TIGR4.,Yu WL, Jiang YL, Pikis A, Cheng W, Bai XH, Ren YM, Thompson J, Zhou CZ, Chen Y J Biol Chem. 2013 May 24;288(21):14949-58. doi: 10.1074/jbc.M113.454751. Epub, 2013 Apr 11. PMID:23580646<ref>PMID:23580646</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4ipn" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
The complex structure of 6-phospho-beta-glucosidase BglA-2 with thiocellobiose-6P from Streptococcus pneumoniae
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Categories: Large Structures | Streptococcus pneumoniae TIGR4 | Andreas P | Bai XH | Chen YX | Cheng W | Jiang YL | Ren YM | Thompsonn J | Yu WL | Zhou CZ
