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1j1n

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{{STRUCTURE_1j1n| PDB=1j1n | SCENE= }}
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'''Structure Analysis of AlgQ2, A Macromolecule(Alginate)-Binding Periplasmic Protein Of Sphingomonas Sp. A1., Complexed with an Alginate Tetrasaccharide'''
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===Structure Analysis of AlgQ2, A Macromolecule(Alginate)-Binding Periplasmic Protein Of Sphingomonas Sp. A1., Complexed with an Alginate Tetrasaccharide===
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==Overview==
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Sphingomonas sp. A1 possesses a high molecular weight (HMW) alginate uptake system composed of a novel pit formed on the cell surface and a pit-dependent ATP-binding cassette (ABC) transporter in the inner membrane. The transportation of HMW alginate from the pit to the ABC transporter is mediated by the periplasmic HMW alginate-binding proteins AlgQ1 and AlgQ2. We determined the crystal structure of AlgQ2 complexed with an alginate tetrasaccharide using an alginate-free (apo) form as a search model and refined it at 1.6-A resolution. One tetrasaccharide was found between the N and C-terminal domains, which are connected by three extended hinge loops. The tetrasaccharide complex took on a closed domain form, in contrast to the open domain form of the apo form. The tetrasaccharide was bound in the cleft between the domains through van der Waals interactions and the formation of hydrogen bonds. Among the four sugar residues, the nonreducing end residue was located at the bottom of the cleft and exhibited the largest number of interactions with the surrounding amino acid residues, suggesting that AlgQ2 mainly recognizes and binds to the nonreducing part of a HMW alginate and delivers the polymer to the ABC transporter through conformational changes (open and closed forms) of the two domains.
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(as it appears on PubMed at http://www.pubmed.gov), where 12486124 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12486124}}
==About this Structure==
==About this Structure==
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[[Category: Murata, K.]]
[[Category: Murata, K.]]
[[Category: Alginate]]
[[Category: Alginate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:41:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:21:55 2008''

Revision as of 11:21, 1 July 2008

Image:1j1n.png

Template:STRUCTURE 1j1n

Structure Analysis of AlgQ2, A Macromolecule(Alginate)-Binding Periplasmic Protein Of Sphingomonas Sp. A1., Complexed with an Alginate Tetrasaccharide

Template:ABSTRACT PUBMED 12486124

About this Structure

1J1N is a Single protein structure of sequence from Sphingomonas sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1, complexed with an alginate tetrasaccharide at 1.6-A resolution., Mishima Y, Momma K, Hashimoto W, Mikami B, Murata K, J Biol Chem. 2003 Feb 21;278(8):6552-9. Epub 2002 Dec 16. PMID:12486124

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