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1j2n

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{{STRUCTURE_1j2n| PDB=1j2n | SCENE= }}
{{STRUCTURE_1j2n| PDB=1j2n | SCENE= }}
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'''Solution structure of CPI-17(22-120) T38D'''
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===Solution structure of CPI-17(22-120) T38D===
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==Overview==
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We present solution NMR structures for wild-type and mutated forms of CPI-17, a phosphoinhibitor for protein phosphatase 1. Phosphorylation of Thr38 of CPI-17 produces a &gt;1000-fold increase in inhibitory potency for myosin phosphatase. We compared the 1H-15N heteronuclear single quantum coherence spectroscopy (HSQC) chemical shifts of wild-type CPI-17, partially phosphorylated CPI-17 and CPI-17 with Thr38 replaced with Asp to introduce a negative charge. There was a switch in the protein conformation due to either Asp substitution or phosphorylation, so we determined the solution NMR structure of the CPI-17 T38D mutant as a model for the active (phospho-) conformation. The structures reveal a molecular switch in conformation that involves the rotation of two of the four helices in the four helix bundle. Despite this conformational switch, there was little increase in the inhibitory potency with T38D. We propose that for this inhibitor, a negative charge at residue 38 is sufficient to trigger an active conformation, but a phosphoryl group is required for full inhibitory potency against protein phosphatase-1.
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{{ABSTRACT_PUBMED_12595264}}
==About this Structure==
==About this Structure==
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1J2N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2N OCA].
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1J2N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2N OCA].
==Reference==
==Reference==
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[[Category: Takada, R.]]
[[Category: Takada, R.]]
[[Category: Helix bundle]]
[[Category: Helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:43:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:24:31 2008''

Revision as of 11:24, 1 July 2008

Image:1j2n.png

Template:STRUCTURE 1j2n

Solution structure of CPI-17(22-120) T38D

Template:ABSTRACT PUBMED 12595264

About this Structure

1J2N is a Single protein structure of sequence from Sus scrofa. Full experimental information is available from OCA.

Reference

Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation-site threonine residue., Ohki S, Eto M, Shimizu M, Takada R, Brautigan DL, Kainosho M, J Mol Biol. 2003 Mar 7;326(5):1539-47. PMID:12595264

Page seeded by OCA on Tue Jul 1 14:24:31 2008

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