1j3y

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{{STRUCTURE_1j3y| PDB=1j3y | SCENE= }}
{{STRUCTURE_1j3y| PDB=1j3y | SCENE= }}
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'''Direct observation of photolysis-induced tertiary structural changes in human hemoglobin; Crystal structure of alpha(Fe)-beta(Ni) hemoglobin (laser photolysed)'''
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===Direct observation of photolysis-induced tertiary structural changes in human hemoglobin; Crystal structure of alpha(Fe)-beta(Ni) hemoglobin (laser photolysed)===
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==Overview==
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Human Hb, an alpha2beta2 tetrameric oxygen transport protein that switches from a T (tense) to an R (relaxed) quaternary structure during oxygenation, has long served as a model for studying protein allostery in general. Time-resolved spectroscopic measurements after photodissociation of CO-liganded Hb have played a central role in exploring both protein dynamical responses and molecular cooperativity, but the direct visualization and the structural consequences of photodeligation have not yet been reported. Here we present an x-ray study of structural changes induced by photodissociation of half-liganded T-state and fully liganded R-state human Hb at cryogenic temperatures (25-35 K). On photodissociation of CO, structural changes involving the heme and the F-helix are more marked in the alpha subunit than in the beta subunit, and more subtle in the R state than in the T state. Photodeligation causes a significant sliding motion of the T-state beta heme. Our results establish that the structural basis of the low affinity of the T state is radically different between the subunits, because of differences in the packing and chemical tension at the hemes.
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The line below this paragraph, {{ABSTRACT_PUBMED_12773618}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12773618 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12773618}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Tertiary structure change]]
[[Category: Tertiary structure change]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:46:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:28:05 2008''

Revision as of 11:28, 1 July 2008

Image:1j3y.png

Template:STRUCTURE 1j3y

Direct observation of photolysis-induced tertiary structural changes in human hemoglobin; Crystal structure of alpha(Fe)-beta(Ni) hemoglobin (laser photolysed)

Template:ABSTRACT PUBMED 12773618

About this Structure

1J3Y is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Direct observation of photolysis-induced tertiary structural changes in hemoglobin., Adachi S, Park SY, Tame JR, Shiro Y, Shibayama N, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7039-44. Epub 2003 May 28. PMID:12773618

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