4jmr
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4jmr]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_foamy_virus Human foamy virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JMR FirstGlance]. <br> | <table><tr><td colspan='2'>[[4jmr]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_foamy_virus Human foamy virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JMR FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jmr OCA], [https://pdbe.org/4jmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jmr RCSB], [https://www.ebi.ac.uk/pdbsum/4jmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jmr ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jmr OCA], [https://pdbe.org/4jmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jmr RCSB], [https://www.ebi.ac.uk/pdbsum/4jmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jmr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/GAG_FOAMV GAG_FOAMV] Involved in capsid formation and genome binding. Shortly after infection, interaction between incoming particle-associated Gag proteins and host dynein allows centrosomal targeting of the viral genome (associated to Gag), prior to nucleus translocation and integration into host genome.<ref>PMID:16160174</ref> <ref>PMID:12857789</ref> | [https://www.uniprot.org/uniprot/GAG_FOAMV GAG_FOAMV] Involved in capsid formation and genome binding. Shortly after infection, interaction between incoming particle-associated Gag proteins and host dynein allows centrosomal targeting of the viral genome (associated to Gag), prior to nucleus translocation and integration into host genome.<ref>PMID:16160174</ref> <ref>PMID:12857789</ref> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The Spumaretrovirinae, or foamyviruses (FVs) are complex retroviruses that infect many species of monkey and ape. Although FV infection is apparently benign, trans-species zoonosis is commonplace and has resulted in the isolation of the Prototypic Foamy Virus (PFV) from human sources and the potential for germ-line transmission. Despite little sequence homology, FV and orthoretroviral Gag proteins perform equivalent functions, including genome packaging, virion assembly, trafficking and membrane targeting. In addition, PFV Gag interacts with the FV Envelope (Env) protein to facilitate budding of infectious particles. Presently, there is a paucity of structural information with regards FVs and it is unclear how disparate FV and orthoretroviral Gag molecules share the same function. Therefore, in order to probe the functional overlap of FV and orthoretroviral Gag and learn more about FV egress and replication we have undertaken a structural, biophysical and virological study of PFV-Gag. We present the crystal structure of a dimeric amino terminal domain from PFV, Gag-NtD, both free and in complex with the leader peptide of PFV Env. The structure comprises a head domain together with a coiled coil that forms the dimer interface and despite the shared function it is entirely unrelated to either the capsid or matrix of Gag from other retroviruses. Furthermore, we present structural, biochemical and virological data that reveal the molecular details of the essential Gag-Env interaction and in addition we also examine the specificity of Trim5alpha restriction of PFV. These data provide the first information with regards to FV structural proteins and suggest a model for convergent evolution of gag genes where structurally unrelated molecules have become functionally equivalent. | ||
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| - | A Unique Spumavirus Gag N-terminal Domain with Functional Properties of Orthoretroviral Matrix and Capsid.,Goldstone DC, Flower TG, Ball NJ, Sanz-Ramos M, Yap MW, Ogrodowicz RW, Stanke N, Reh J, Lindemann D, Stoye JP, Taylor IA PLoS Pathog. 2013 May;9(5):e1003376. doi: 10.1371/journal.ppat.1003376. Epub 2013, May 9. PMID:23675305<ref>PMID:23675305</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4jmr" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
Current revision
A unique spumavirus gag N-terminal domain with functional properties of orthoretroviral Matrix and Capsid
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