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1j7t

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{{STRUCTURE_1j7t| PDB=1j7t | SCENE= }}
{{STRUCTURE_1j7t| PDB=1j7t | SCENE= }}
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'''Complex between Paromomycin and the 16S-rRNA A-site at 2.5 A resolution'''
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===Complex between Paromomycin and the 16S-rRNA A-site at 2.5 A resolution===
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==Overview==
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BACKGROUND: Aminoglycoside antibiotics interfere with translation in both gram-positive and gram-negative bacteria by binding to the tRNA decoding A site of the 16S ribosomal RNA. RESULTS: Crystals of complexes between oligoribonucleotides incorporating the sequence of the ribosomal A site of Escherichia coli and the aminoglycoside paromomycin have been solved at 2.5 A resolution. Each RNA fragment contains two A sites inserted between Watson-Crick pairs. The paromomycin molecules interact in an enlarged deep groove created by two bulging and one unpaired adenines. In both sites, hydroxyl and ammonium side chains of the antibiotic form 13 direct hydrogen bonds to bases and backbone atoms of the A site. In the best-defined site, 8 water molecules mediate 12 other hydrogen bonds between the RNA and the antibiotics. Ring I of paromomycin stacks over base G1491 and forms pseudo-Watson-Crick contacts with A1408. Both the hydroxyl group and one ammonium group of ring II form direct and water-mediated hydrogen bonds to the U1495oU1406 pair. The bulging conformation of the two adenines A1492 and A1493 is stabilized by hydrogen bonds between phosphate oxygens and atoms of rings I and II. The hydrophilic sites of the bulging A1492 and A1493 contact the shallow groove of G=C pairs in a symmetrical complex. CONCLUSIONS: Water molecules participate in the binding specificity by exploiting the antibiotic hydration shell and the typical RNA water hydration patterns. The observed contacts rationalize the protection, mutation, and resistance data. The crystal packing mimics the intermolecular contacts induced by aminoglycoside binding in the ribosome.
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The line below this paragraph, {{ABSTRACT_PUBMED_11587639}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11587639 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11587639}}
==About this Structure==
==About this Structure==
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[[Category: Rna-aminoglycoside interaction]]
[[Category: Rna-aminoglycoside interaction]]
[[Category: Uou pair]]
[[Category: Uou pair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:53:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:36:24 2008''

Revision as of 11:36, 1 July 2008

Image:1j7t.png

Template:STRUCTURE 1j7t

Complex between Paromomycin and the 16S-rRNA A-site at 2.5 A resolution

Template:ABSTRACT PUBMED 11587639

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of paromomycin docked into the eubacterial ribosomal decoding A site., Vicens Q, Westhof E, Structure. 2001 Aug;9(8):647-58. PMID:11587639

Page seeded by OCA on Tue Jul 1 14:36:24 2008

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