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4kfr
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4kfr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_turreted_icosahedral_virus_2 Sulfolobus turreted icosahedral virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KFR FirstGlance]. <br> | <table><tr><td colspan='2'>[[4kfr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_turreted_icosahedral_virus_2 Sulfolobus turreted icosahedral virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KFR FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.956Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kfr OCA], [https://pdbe.org/4kfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kfr RCSB], [https://www.ebi.ac.uk/pdbsum/4kfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kfr ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kfr OCA], [https://pdbe.org/4kfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kfr RCSB], [https://www.ebi.ac.uk/pdbsum/4kfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kfr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/D5IEZ9_9VIRU D5IEZ9_9VIRU] | [https://www.uniprot.org/uniprot/D5IEZ9_9VIRU D5IEZ9_9VIRU] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Biochemical reactions powered by ATP hydrolysis are fundamental for the movement of molecules and cellular structures. One such reaction is the encapsidation of the dsDNA genome of an icosahedrally-symmetric virus into a preformed procapsid with the help of a genome translocating NTPase. Such NTPases have been characterized in detail from both RNA and tailed DNA viruses. We present four crystal structures and the biochemical activity of a thermophilic NTPase, B204, from the non-tailed, membrane-containing, hyperthermo-acidophilic archaeal dsDNA virus Sulfolobus turreted icosahedral virus 2. These are the first structures of a genome packaging NTPase from a non-tailed, dsDNA virus with an archaeal host. The four structures highlight the catalytic cycle of B204, pinpointing the molecular movement between substrate-bound (open) and empty (closed) active sites. The protein is shown to bind both single-stranded and double-stranded nucleic acids, and to have an optimum activity at 80 degrees C and pH 4.5. The overall fold of B204 places it in the FtsK-HerA superfamily of P-loop ATPases, whose cellular and viral members have been suggested to share a common DNA-translocating mechanism. | ||
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| - | The structure of the NTPase that powers DNA packaging into Sulfolobus turreted icosahedral virus 2.,Happonen LJ, Oksanen E, Liljeroos L, Goldman A, Kajander T, Butcher SJ J Virol. 2013 May 22. PMID:23698307<ref>PMID:23698307</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4kfr" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Structure of the genome packaging NTPase B204 from Sulfolobus turreted icosahedral virus 2 in complex with sulfate
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