8dwf

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Current revision (05:25, 9 August 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8dwf is ON HOLD until Paper Publication
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==Glycosylase MutY variant E43S in complex with DNA containing d(8-oxo-G) paired with substrate adenine==
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<StructureSection load='8dwf' size='340' side='right'caption='[[8dwf]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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Authors: Russelburg, L.P., Demir, M., David, S.S., Horvath, M.P.
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8dwf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DWF FirstGlance]. <br>
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Description: Glycosylase MutY variant E43S in complex with DNA containing d(8-oxo-G) paired with substrate adenine
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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[[Category: Unreleased Structures]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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[[Category: Horvath, M.P]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dwf OCA], [https://pdbe.org/8dwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dwf RCSB], [https://www.ebi.ac.uk/pdbsum/8dwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dwf ProSAT]</span></td></tr>
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[[Category: Demir, M]]
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</table>
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[[Category: David, S.S]]
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== Function ==
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[[Category: Russelburg, L.P]]
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[https://www.uniprot.org/uniprot/MUTY_GEOSE MUTY_GEOSE] Base excision repair (BER) glycosylase that initiates repair of A:oxoG to C:G by removing the inappropriately paired adenine base from the DNA backbone, generating an abasic site product (PubMed:25995449) (PubMed:14961129). 8-oxoguanine (oxoG) is a genotoxic DNA lesion resulting from oxidation of guanine; this residue is misread by replicative DNA polymerases, that insert adenine instead of cytosine opposite the oxidized damaged base. Shows a powerful dicrimination of A versus C, since it does not cleave cytosine in oxoG:C pairs (PubMed:25995449). May also be able to remove adenine from A:G mispairs, although this activity may not be physiologically relevant (PubMed:14961129).<ref>PMID:25995449</ref> <ref>PMID:14961129</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: David SS]]
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[[Category: Demir M]]
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[[Category: Horvath MP]]
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[[Category: Russelburg LP]]

Current revision

Glycosylase MutY variant E43S in complex with DNA containing d(8-oxo-G) paired with substrate adenine

PDB ID 8dwf

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