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| - | [[Image:1j9o.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1j9o| PDB=1j9o | SCENE= }} | | {{STRUCTURE_1j9o| PDB=1j9o | SCENE= }} |
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| - | '''SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN'''
| + | ===SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN=== |
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| - | ==Overview==
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| - | Lymphotactin, the sole identified member of the C class of chemokines, specifically attracts T lymphocytes and natural killer cells. This 93-residue protein lacks 2 of the 4 conserved cysteine residues characteristic of the other 3 classes of chemokines and possesses an extended carboxyl terminus, which is required for chemotactic activity. We have determined the three-dimensional solution structure of recombinant human lymphotactin by NMR spectroscopy. Under the conditions used for the structure determination, lymphotactin was predominantly monomeric; however, pulsed field gradient NMR self-diffusion measurements and analytical ultracentrifugation revealed evidence of dimer formation. Sequence-specific chemical shift assignments were determined through analysis of two- and three-dimensional NMR spectra of (15)N- and (13)C/(15)N-enriched protein samples. Input for the torsion angle dynamics calculations used in determining the structure included 1258 unique NOE-derived distance constraints and 60 dihedral angle constraints obtained from chemical-shift-based searching of a protein conformational database. The ensemble of 20 structures chosen to represent the structure had backbone and heavy atom rms deviations of 0.46 +/- 0.11 and 1.02 +/- 0.14 A, respectively. The results revealed that human lymphotactin adopts the conserved chemokine fold, which is characterized by a three-stranded antiparallel beta-sheet and a C-terminal alpha-helix. Two regions are dynamically disordered as evidenced by (1)H and (13)C chemical shifts and [(15)N]-(1)H NOEs: residues 1-9 of the amino terminus and residues 69-93 of the C-terminal extension. A functional role for the C-terminal extension, which is unique to lymphotactin, remains to be elucidated.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11601972}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11601972 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11601972}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1J9O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9O OCA]. | + | 1J9O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9O OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Volkman, B F.]] | | [[Category: Volkman, B F.]] |
| | [[Category: Chemokine]] | | [[Category: Chemokine]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:57:20 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:44:41 2008'' |
Revision as of 11:44, 1 July 2008
Template:STRUCTURE 1j9o
SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN
Template:ABSTRACT PUBMED 11601972
About this Structure
1J9O is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
Monomeric solution structure of the prototypical 'C' chemokine lymphotactin., Kuloglu ES, McCaslin DR, Kitabwalla M, Pauza CD, Markley JL, Volkman BF, Biochemistry. 2001 Oct 23;40(42):12486-96. PMID:11601972
Page seeded by OCA on Tue Jul 1 14:44:41 2008
