1jba

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{{STRUCTURE_1jba| PDB=1jba | SCENE= }}
{{STRUCTURE_1jba| PDB=1jba | SCENE= }}
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'''UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND'''
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===UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND===
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==Overview==
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Guanylyl cyclase activating protein-2 (GCAP-2) is a Ca2+-sensitive regulator of phototransduction in retinal photoreceptor cells. GCAP-2 activates retinal guanylyl cyclases at low Ca2+ concentration (&lt;100 nM) and inhibits them at high Ca2+ (&gt;500 nM). The light-induced lowering of the Ca2+ level from approximately 500 nM in the dark to approximately 50 nM following illumination is known to play a key role in visual recovery and adaptation. We report here the three-dimensional structure of unmyristoylated GCAP-2 with three bound Ca2+ ions as determined by nuclear magnetic resonance spectroscopy of recombinant, isotopically labeled protein. GCAP-2 contains four EF-hand motifs arranged in a compact tandem array like that seen previously in recoverin. The root mean square deviation of the main chain atoms in the EF-hand regions is 2.2 A in comparing the Ca2+-bound structures of GCAP-2 and recoverin. EF-1, as in recoverin, does not bind calcium because it contains a disabling Cys-Pro sequence. GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addition to EF-2 and EF-3. A prominent exposed patch of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49, Tyr81, Val82, Leu85, and Leu89) may serve as a target-binding site for the transmission of calcium signals to guanylyl cyclase.
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{{ABSTRACT_PUBMED_10383444}}
==About this Structure==
==About this Structure==
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1JBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA].
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1JBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA].
==Reference==
==Reference==
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Guanylyl cyclase regulation]]
[[Category: Guanylyl cyclase regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 19:57:34 2008''

Revision as of 16:57, 1 July 2008

Image:1jba.png

Template:STRUCTURE 1jba

UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND

Template:ABSTRACT PUBMED 10383444

About this Structure

1JBA is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases., Ames JB, Dizhoor AM, Ikura M, Palczewski K, Stryer L, J Biol Chem. 1999 Jul 2;274(27):19329-37. PMID:10383444

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