1hu3
From Proteopedia
(New page: 200px<br /> <applet load="1hu3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hu3, resolution 2.37Å" /> '''MIDDLE DOMAIN OF HU...) |
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| - | [[Image:1hu3.gif|left|200px]]<br /> | + | [[Image:1hu3.gif|left|200px]]<br /><applet load="1hu3" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1hu3" size=" | + | |
caption="1hu3, resolution 2.37Å" /> | caption="1hu3, resolution 2.37Å" /> | ||
'''MIDDLE DOMAIN OF HUMAN EIF4GII'''<br /> | '''MIDDLE DOMAIN OF HUMAN EIF4GII'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray structure of the phylogenetically conserved middle portion of | + | The X-ray structure of the phylogenetically conserved middle portion of human eukaryotic initiation factor (eIF) 4GII has been determined at 2.4 A resolution, revealing a crescent-shaped domain consisting of ten alpha helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain suffices for 48S ribosomal complex formation with a picornaviral RNA internal ribosome entry site (IRES). Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of this essential component of the translation initiation machinery that, respectively, bind eIF4A and a picornaviral IRES. The structural and biochemical results provide mechanistic insights into both cap-dependent and cap-independent translation initiation. |
==About this Structure== | ==About this Structure== | ||
| - | 1HU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1HU3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HU3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Hellen, C | + | [[Category: Hellen, C U.T.]] |
[[Category: Lomakin, I.]] | [[Category: Lomakin, I.]] | ||
[[Category: Marcotrigiano, J.]] | [[Category: Marcotrigiano, J.]] | ||
| - | [[Category: Pestova, T | + | [[Category: Pestova, T V.]] |
[[Category: Sonenberg, N.]] | [[Category: Sonenberg, N.]] | ||
[[Category: eif4g]] | [[Category: eif4g]] | ||
[[Category: heat repeat]] | [[Category: heat repeat]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:04:51 2008'' |
Revision as of 11:04, 21 February 2008
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MIDDLE DOMAIN OF HUMAN EIF4GII
Overview
The X-ray structure of the phylogenetically conserved middle portion of human eukaryotic initiation factor (eIF) 4GII has been determined at 2.4 A resolution, revealing a crescent-shaped domain consisting of ten alpha helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain suffices for 48S ribosomal complex formation with a picornaviral RNA internal ribosome entry site (IRES). Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of this essential component of the translation initiation machinery that, respectively, bind eIF4A and a picornaviral IRES. The structural and biochemical results provide mechanistic insights into both cap-dependent and cap-independent translation initiation.
About this Structure
1HU3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery., Marcotrigiano J, Lomakin IB, Sonenberg N, Pestova TV, Hellen CU, Burley SK, Mol Cell. 2001 Jan;7(1):193-203. PMID:11172724
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