1jdm

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[[Image:1jdm.gif|left|200px]]
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{{STRUCTURE_1jdm| PDB=1jdm | SCENE= }}
{{STRUCTURE_1jdm| PDB=1jdm | SCENE= }}
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'''NMR Structure of Sarcolipin'''
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===NMR Structure of Sarcolipin===
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==Overview==
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Sarcolipin (SLN) is a 31 amino acid integral membrane protein that regulates Ca-ATPase activity in skeletal muscle. Here, we report the three-dimensional structure and topology of synthetic SLN in lipid environments, as determined by solution and solid-state NMR spectroscopy. 2D solution NMR experiments were performed on SLN solubilized in sodium dodecyl sulfate (SDS) micelles. We found that SLN adopts a highly defined alpha-helical conformation from F9 through R27, with a backbone RMSD of 0.65 A and a side chain RMSD of 1.66 A. The N-terminus (M1 through L8) and the C-terminus (S28 through Y31) are mostly unstructured. The orientation of the SLN was determined using one-dimensional (15)N NMR solid-state spectroscopy. The protein was incorporated into phospholipid bilayers prepared from a mixture of 1,2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. The (15)N chemical shift solid-state spectra from selectively labeled SLN samples indicate that SLN orients perpendicularly to the plane of the membrane bilayers. These results support the proposed mechanism of Ca-ATPase regulation of SLN via protein-protein intramembranous interactions between the highly conserved transmembrane domains of the Ca-ATPase and the conserved transmembrane domain of SLN.
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(as it appears on PubMed at http://www.pubmed.gov), where 11781085 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11781085}}
==About this Structure==
==About this Structure==
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1JDM is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JDM OCA].
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1JDM is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JDM OCA].
==Reference==
==Reference==
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[[Category: Veglia, G.]]
[[Category: Veglia, G.]]
[[Category: Helix]]
[[Category: Helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:05:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:04:53 2008''

Revision as of 17:04, 1 July 2008

Image:1jdm.png

Template:STRUCTURE 1jdm

NMR Structure of Sarcolipin

Template:ABSTRACT PUBMED 11781085

About this Structure

1JDM is a Single protein structure. Full experimental information is available from OCA.

Reference

Structure and orientation of sarcolipin in lipid environments., Mascioni A, Karim C, Barany G, Thomas DD, Veglia G, Biochemistry. 2002 Jan 15;41(2):475-82. PMID:11781085

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