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Publication Abstract from PubMed

Owing to their outstanding catalytic properties, enzymes represent powerful tools for carrying out a wide range of (bio)chemical transformations with high proficiency. In this context, enzymes with high biocatalytic promiscuity are somewhat neglected. Here, we demonstrate that a meticulous modification of a synthetic shell that surrounds an immobilized enzyme possessing broad substrate specificity allows the resulting nanobiocatalyst to be endowed with enantioselective properties while maintaining a high level of substrate promiscuity. Our results show that control of the enzyme nano-environment enables tuning of both substrate specificity and enantioselectivity. Further, we demonstrate that our strategy of enzyme supramolecular engineering allows the enzyme to be endowed with markedly enhanced stability in an organic solvent (i.e., acetonitrile). The versatility of the method was assessed with two additional substrate-promiscuous and structurally different enzymes, for which improvements in enantioselectivity and stability were confirmed. We expect this method to promote the use of supramolecularly engineered promiscuous enzymes in industrially relevant biocatalytic processes.

Tuning the Properties of Natural Promiscuous Enzymes by Engineering Their Nano-environment.,Giunta CI, Cea-Rama I, Alonso S, Briand ML, Bargiela R, Coscolin C, Corvini PF, Ferrer M, Sanz-Aparicio J, Shahgaldian P ACS Nano. 2020 Dec 11. doi: 10.1021/acsnano.0c08716. PMID:33306346^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.