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1jft

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[[Image:1jft.gif|left|200px]]
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{{STRUCTURE_1jft| PDB=1jft | SCENE= }}
{{STRUCTURE_1jft| PDB=1jft | SCENE= }}
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'''PURINE REPRESSOR MUTANT-HYPOXANTHINE-PURF OPERATOR COMPLEX'''
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===PURINE REPRESSOR MUTANT-HYPOXANTHINE-PURF OPERATOR COMPLEX===
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==Overview==
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The crystal structures of corepressor-bound and free Escherichia coli purine repressor (PurR) have delineated the roles of several residues in corepressor binding and specificity and the intramolecular signal transduction (allosterism) of this LacI/GalR family member. From these structures, residue W147 was implicated as a key component of the allosteric response, but in many members of the LacI/GalR family, position 147 is occupied by an arginine. To understand the role of this tryptophan at position 147, three proteins, substituted by phenylalanine (W147F), alanine (W147A), or arginine (W147R), were constructed and characterized in vivo and in vitro, and their structures were determined. W147F displays a decreased affinity for corepressor and is a poor repressor in vivo. W147A and W147R, on the other hand, are super repressors and bind corepressor 13.6 and 7.9 times more tightly, respectively, than wild-type. Each mutant PurR-hypoxanthine-purF operator holo complex crystallizes isomorphously to wild-type. Whereas the apo corepressor binding domain (CBD) of W147F crystallizes under those conditions used for the wild-type protein, neither the apo CBD of W147R nor W147A crystallizes, although screened extensively for new crystal forms. Structures of the holo repressor mutants have been solved to resolutions between 2.5 and 2.9 A, and the structure of the apo CBD of W147F has been solved to 2.4 A resolution. These structures provide insight into the altered biochemical properties and physiological functions of these mutants, which appear to depend on the sometimes subtle preference for one conformation (apo vs holo) over the other.
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(as it appears on PubMed at http://www.pubmed.gov), where 11781089 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11781089}}
==About this Structure==
==About this Structure==
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[[Category: Repressor]]
[[Category: Repressor]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:10:57 2008''

Revision as of 17:10, 1 July 2008

Image:1jft.png

Template:STRUCTURE 1jft

PURINE REPRESSOR MUTANT-HYPOXANTHINE-PURF OPERATOR COMPLEX

Template:ABSTRACT PUBMED 11781089

About this Structure

1JFT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor., Huffman JL, Lu F, Zalkin H, Brennan RG, Biochemistry. 2002 Jan 15;41(2):511-20. PMID:11781089

Page seeded by OCA on Tue Jul 1 20:10:57 2008

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