4mub
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MUB FirstGlance]. <br> | <table><tr><td colspan='2'>[[4mub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MUB FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=OAQ:{(2S)-7-NITRO-2-[(PROPAN-2-YLAMINO)METHYL]-1,2,3,4-TETRAHYDROQUINOLIN-6-YL}METHANOL'>OAQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=OAQ:{(2S)-7-NITRO-2-[(PROPAN-2-YLAMINO)METHYL]-1,2,3,4-TETRAHYDROQUINOLIN-6-YL}METHANOL'>OAQ</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mub OCA], [https://pdbe.org/4mub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mub RCSB], [https://www.ebi.ac.uk/pdbsum/4mub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mub ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mub OCA], [https://pdbe.org/4mub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mub RCSB], [https://www.ebi.ac.uk/pdbsum/4mub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mub ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/G4VLE5_SCHMA G4VLE5_SCHMA] | [https://www.uniprot.org/uniprot/G4VLE5_SCHMA G4VLE5_SCHMA] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Oxamniquine resistance evolved in the human blood fluke (Schistosoma mansoni) in Brazil in the 1970s. We crossed parental parasites differing ~500-fold in drug response, determined drug sensitivity and marker segregation in clonally derived second-generation progeny, and identified a single quantitative trait locus (logarithm of odds = 31) on chromosome 6. A sulfotransferase was identified as the causative gene by using RNA interference knockdown and biochemical complementation assays, and we subsequently demonstrated independent origins of loss-of-function mutations in field-derived and laboratory-selected resistant parasites. These results demonstrate the utility of linkage mapping in a human helminth parasite, while crystallographic analyses of protein-drug interactions illuminate the mode of drug action and provide a framework for rational design of oxamniquine derivatives that kill both S. mansoni and S. haematobium, the two species responsible for >99% of schistosomiasis cases worldwide. | ||
| - | |||
| - | Genetic and molecular basis of drug resistance and species-specific drug action in schistosome parasites.,Valentim CL, Cioli D, Chevalier FD, Cao X, Taylor AB, Holloway SP, Pica-Mattoccia L, Guidi A, Basso A, Tsai IJ, Berriman M, Carvalho-Queiroz C, Almeida M, Aguilar H, Frantz DE, Hart PJ, LoVerde PT, Anderson TJ Science. 2013 Dec 13;342(6164):1385-9. doi: 10.1126/science.1243106. Epub 2013, Nov 21. PMID:24263136<ref>PMID:24263136</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4mub" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]] | *[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Schistosoma mansoni (Blood Fluke) Sulfotransferase/Oxamniquine Complex
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Categories: Large Structures | Schistosoma mansoni | Aguilar H | Almeida M | Anderson TJC | Basso A | Berriman M | Cao X | Carvalho-Queiroz C | Chevalier FD | Cioli D | Frantz DE | Guidi A | Hart PJ | Holloway SP | LoVerde PT | Pica-Mattoccia L | Taylor AB | Tsai IJ | Valentim CLL
