1jl1

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[[Image:1jl1.jpg|left|200px]]
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{{STRUCTURE_1jl1| PDB=1jl1 | SCENE= }}
{{STRUCTURE_1jl1| PDB=1jl1 | SCENE= }}
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'''D10A E. coli ribonuclease HI'''
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===D10A E. coli ribonuclease HI===
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==Overview==
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Escherichia coli RNase HI is a well-characterized model system for protein folding and stability. Controlling protein stability is critical for both natural proteins and for the development of engineered proteins that function under extreme conditions. We have used native-state hydrogen exchange on a variant containing the stabilizing mutation Asp10 to alanine in order to determine its residue-specific stabilities. On average, the DeltaG(unf) value for each residue was increased by 2-3 kcal/mol, resulting in a lower relative population of partially unfolded forms. Though increased in stability by a uniform factor, D10A shows a distribution of stabilities in its secondary structural units that is similar to that of E. coli RNase H, but not the closely related protein from Thermus thermophilus. Hence, the simple mutation used to stabilize the enzyme does not recreate the balance of conformational flexibility evolved in the thermophilic protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 11734003 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11734003}}
==About this Structure==
==About this Structure==
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[[Category: Rnase hi]]
[[Category: Rnase hi]]
[[Category: Thermostability]]
[[Category: Thermostability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:21:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:24:32 2008''

Revision as of 17:24, 1 July 2008

Image:1jl1.png

Template:STRUCTURE 1jl1

D10A E. coli ribonuclease HI

Template:ABSTRACT PUBMED 11734003

About this Structure

1JL1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Native-state energetics of a thermostabilized variant of ribonuclease HI., Goedken ER, Marqusee S, J Mol Biol. 2001 Dec 7;314(4):863-71. PMID:11734003

Page seeded by OCA on Tue Jul 1 20:24:32 2008

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