Journal:MicroPubl Biol:000670
From Proteopedia
(Difference between revisions)
| Line 6: | Line 6: | ||
Genomic sequencing of cotton species identified thousands of uncharacterized proteins (Chen ''et al''., 2020<ref name='Chen'>PMID: 32313247</ref>). One encoded by the gene LOC107943309, termed ‘light-dependent short hypocotyls 4-like’ (NCBI: XP_016732548, Uniprot: A0A1U8MC48) or GhLSH4L-A0A1U8MC48, was shown to be part of the ALOG (Arabidopsis LSH1 (light-dependent short hypocotyl) and Oryza G1) family and contains the necessary sequence and structure for localization to the nucleus and subsequent DNA-binding. | Genomic sequencing of cotton species identified thousands of uncharacterized proteins (Chen ''et al''., 2020<ref name='Chen'>PMID: 32313247</ref>). One encoded by the gene LOC107943309, termed ‘light-dependent short hypocotyls 4-like’ (NCBI: XP_016732548, Uniprot: A0A1U8MC48) or GhLSH4L-A0A1U8MC48, was shown to be part of the ALOG (Arabidopsis LSH1 (light-dependent short hypocotyl) and Oryza G1) family and contains the necessary sequence and structure for localization to the nucleus and subsequent DNA-binding. | ||
| - | The ALOG family is largely found in flowering plants with over 80% belonging to dicots (Iyer and Aravind, 2012<ref name='Aravind'>PMID: 23146749</ref>). Members of the ALOG family appear to be involved in tissue and organ development in plants with evidence that some function as transcription factors, regulating the expression of particular sets of genes. The 125-residue ALOG domain may be derived from the N-terminal DNA-binding domain (DBD) of viral recombinases and integrases, proteins that rearrange DNA segments (Iyer and Aravind, 2012<ref name='Aravind'>PMID: 23146749</ref>). One of these proteins, CRE recombinase, has been crystalized bound to DNA (PDB [[1crx]], Guo ''et al''., | + | The ALOG family is largely found in flowering plants with over 80% belonging to dicots (Iyer and Aravind, 2012<ref name='Aravind'>PMID: 23146749</ref>). Members of the ALOG family appear to be involved in tissue and organ development in plants with evidence that some function as transcription factors, regulating the expression of particular sets of genes. The 125-residue ALOG domain may be derived from the N-terminal DNA-binding domain (DBD) of viral recombinases and integrases, proteins that rearrange DNA segments (Iyer and Aravind, 2012<ref name='Aravind'>PMID: 23146749</ref>). One of these proteins, CRE recombinase, has been crystalized bound to DNA (PDB [[1crx]], Guo ''et al''., 1997<ref name='Guo'>PMID: 9288963</ref>) through a core four helix-bundle where three helices interact with DNA through the major groove with a fourth helix located near the C-terminal. ALOG domains in plants also have a four helix bundle but are set apart by a Zinc-Ribbon region inserted between helices 2 and 3 that is proposed to chelate a Zinc ion. |
An AlphaFold model of GhLSH4L-A0A1U8MC48 predicted a high level of confidence in the ALOG domain region from amino acid 55 to 193, which consists of a bundle of four alpha helices characteristic of ALOG proteins (Iyer and Aravind, 2012<ref name='Aravind'>PMID: 23146749</ref>). | An AlphaFold model of GhLSH4L-A0A1U8MC48 predicted a high level of confidence in the ALOG domain region from amino acid 55 to 193, which consists of a bundle of four alpha helices characteristic of ALOG proteins (Iyer and Aravind, 2012<ref name='Aravind'>PMID: 23146749</ref>). | ||
Revision as of 13:29, 12 January 2023
| |||||||||||
This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
