NKX2.5 Homeodomain

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=== Protein / Protein Interactions===
=== Protein / Protein Interactions===
The global structure of NKX2.5 is maintained by both hydrophobic and hydrophilic interactions between the three alpha-helices. This is clear when observing the structure of NKX2.5 where <scene name='91/911264/Hydrophilic_and_phobic/1'>coloring is based on hydrophobicity</scene>. Besides these tertiary interactions present in NKX2.5, the protein is known to associate with other transcription factors and regulatory proteins. For example, GATA factors, and the Hand1 transcription factor (both of which are important in cardiogenesis) are known to interact with NKX2.5 <ref> PMID: 9312027 </ref>,<ref> PMID: 21519287 </ref>. Unfortunately, structures are not available for these complexes. Structures have been elucidated for the interaction of <scene name='91/911264/Tbx5/1'>NKX2.5 with the transcription factor TBX5</scene> (T-box5)<ref> PMID: 26926761 </ref>. This interaction appears is thought to be mediated <scene name='91/911264/Tbx5_interactions/1'>through three residues</scene>: Lys 158 of NKX2.5 along with Asp 140 and Pro 142 from TBX5. A complex formed between <scene name='91/911264/Nkx25_and_mef2/1'>NKX2.5 and MEF2</scene> (myocyte enhancer factor 2) has also been elucidated <ref> PMID: 32681840 </ref>.
The global structure of NKX2.5 is maintained by both hydrophobic and hydrophilic interactions between the three alpha-helices. This is clear when observing the structure of NKX2.5 where <scene name='91/911264/Hydrophilic_and_phobic/1'>coloring is based on hydrophobicity</scene>. Besides these tertiary interactions present in NKX2.5, the protein is known to associate with other transcription factors and regulatory proteins. For example, GATA factors, and the Hand1 transcription factor (both of which are important in cardiogenesis) are known to interact with NKX2.5 <ref> PMID: 9312027 </ref>,<ref> PMID: 21519287 </ref>. Unfortunately, structures are not available for these complexes. Structures have been elucidated for the interaction of <scene name='91/911264/Tbx5/1'>NKX2.5 with the transcription factor TBX5</scene> (T-box5)<ref> PMID: 26926761 </ref>. This interaction appears is thought to be mediated <scene name='91/911264/Tbx5_interactions/1'>through three residues</scene>: Lys 158 of NKX2.5 along with Asp 140 and Pro 142 from TBX5. A complex formed between <scene name='91/911264/Nkx25_and_mef2/1'>NKX2.5 and MEF2</scene> (myocyte enhancer factor 2) has also been elucidated <ref> PMID: 32681840 </ref>.
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=== Evolution ===
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== Evolution ==
The homeodomain of NKX2.5 is highly conserved across the animal kingdom <ref name="WJ" />. This can easily be seen in a sequence alignment containing NKX2.5 isoforms across a variety of species. A selection of the Clustal Omega sequence alignment <ref> PMID: 23671338 </ref> shown below outlines the conservation of the homeodomain of NKX2.5.
The homeodomain of NKX2.5 is highly conserved across the animal kingdom <ref name="WJ" />. This can easily be seen in a sequence alignment containing NKX2.5 isoforms across a variety of species. A selection of the Clustal Omega sequence alignment <ref> PMID: 23671338 </ref> shown below outlines the conservation of the homeodomain of NKX2.5.

Revision as of 09:24, 16 January 2023

PDB ID 3rkq

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Proteopedia Page Contributors and Editors (what is this?)

William J Simard, Michal Harel

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