1jmb

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[[Image:1jmb.gif|left|200px]]
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==Overview==
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{{ABSTRACT_11749531}}
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De novo design of proteins provides an attractive approach to uncover the essential features required for their functions. Previously, we described the design and crystal structure determination of a di-Zn(II) complex of "due-ferri-1" (DF1), a protein patterned after the diiron-dimanganese class of redox-active proteins [Lombardi, A.; Summa, C.; Geremia, S.; Randaccio, L.; Pavone, V.; DeGrado, W. F. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 6298-6305]. The overall structure of DF1, which contains a carboxylate-bridged dinuclear metal site, agrees well with the intended design. However, access to this dimetal site is blocked by a pair of hydrophobic leucine residues (L13 and L13'), which prevent facile entry of metal ions and small molecules. We have now taken the next step in the eventual construction of a catalytically active metalloenzyme by engineering an active site cavity into DF1 through the replacement of these two leucine residues with smaller residues. The crystal structure of the dimanganous form of L13A-DF1 indeed shows a substrate access channel to the dimetal center. In the crystal structure, water molecules and a ligating dimethyl sulfoxide molecule, which forms a monatomic bridge between the metal ions, occupy the cavity. Furthermore, the diferric form of a derivative of L13A-DF1, DF2, is shown to bind azide, acetate, and small aromatic molecules.
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==About this Structure==
==About this Structure==
The following page contains interesting information on the relation of 1JMB with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMB OCA].
The following page contains interesting information on the relation of 1JMB with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMB OCA].
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==Reference==
 
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Toward the de novo design of a catalytically active helix bundle: a substrate-accessible carboxylate-bridged dinuclear metal center., Di Costanzo L, Wade H, Geremia S, Randaccio L, Pavone V, DeGrado WF, Lombardi A, J Am Chem Soc. 2001 Dec 26;123(51):12749-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11749531 11749531]
 
[[Category: Designer Proteins]]
[[Category: Designer Proteins]]
[[Category: Costanzo, L Di.]]
[[Category: Costanzo, L Di.]]
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[[Category: Alpha-helical bundle]]
[[Category: Alpha-helical bundle]]
[[Category: Protein design]]
[[Category: Protein design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:24:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:36:45 2008''

Revision as of 14:36, 26 June 2008

Image:1jmb.png

Template:STRUCTURE 1jmb

CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL


Template:ABSTRACT 11749531

About this Structure

The following page contains interesting information on the relation of 1JMB with [Designer Proteins]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:36:45 2008

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