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7xhf

From Proteopedia

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Current revision

Crystal structure of the NTF2L domain of human G3BP1 in complex with the USP10 derived peptide

Structural highlights

7xhf is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.68Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G3BP1_HUMAN May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.^[1] ^[2]

Publication Abstract from PubMed

Stress granules (SGs) are cytoplasmic biomolecular condensates containing proteins and RNAs in response to stress. Ras-GTPase-activating protein binding protein 1 (G3BP1) is a core SG protein. Caprin-1 and ubiquitin specific peptidase 10 (USP10) interact with G3BP1, facilitating and suppressing SG formation, respectively. The crystal structures of the nuclear transport factor 2-like (NTF2L) domain of G3BP1 in complex with the G3BP1-interacting motif (GIM) of Caprin-1 and USP10 show that both GIMs bind to the same hydrophobic pocket of G3BP1. Moreover, both GIMs suppressed the liquid-liquid phase separation (LLPS) of G3BP1, suggesting that Caprin-1 likely facilitates SG formation via other mechanisms. Thus, we dissected various domains of Caprin-1 and investigated their role in LLPS in vitro and SG formation in cells. The C-terminal domain of Caprin-1 underwent spontaneous LLPS, whereas the N-terminal domain and GIM of Caprin-1 suppressed LLPS of G3BP1. The opposing effect of the N- and C-terminal domains of Caprin-1 on SG formation were demonstrated in cells with or without the endogenous Caprin-1. We propose that the N- and C-terminal domains of Caprin-1 regulate SG formation in a "yin and yang" fashion, mediating the dynamic and reversible assembly of SGs.

Yin and yang regulation of stress granules by Caprin-1.,Song D, Kuang L, Yang L, Wang L, Li H, Li X, Zhu Z, Shi C, Zhu H, Gong W Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2207975119. doi: , 10.1073/pnas.2207975119. Epub 2022 Oct 24. PMID:36279435^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Costa M, Ochem A, Staub A, Falaschi A. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway. Nucleic Acids Res. 1999 Feb 1;27(3):817-21. PMID:9889278
↑ Tourriere H, Gallouzi IE, Chebli K, Capony JP, Mouaikel J, van der Geer P, Tazi J. RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization. Mol Cell Biol. 2001 Nov;21(22):7747-60. PMID:11604510 doi:10.1128/MCB.21.22.7747-7760.2001
↑ Song D, Kuang L, Yang L, Wang L, Li H, Li X, Zhu Z, Shi C, Zhu H, Gong W. Yin and yang regulation of stress granules by Caprin-1. Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2207975119. doi: , 10.1073/pnas.2207975119. Epub 2022 Oct 24. PMID:36279435 doi:http://dx.doi.org/10.1073/pnas.2207975119

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7xhf"

Categories: Homo sapiens | Large Structures | Dan S | Weimin G

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[7xhf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHF FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhf OCA], [https://pdbe.org/7xhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhf RCSB], [https://www.ebi.ac.uk/pdbsum/7xhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhf ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhf OCA], [https://pdbe.org/7xhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhf RCSB], [https://www.ebi.ac.uk/pdbsum/7xhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhf ProSAT]</span></td></tr>
 </table>
 == Function ==

7xhf

From Proteopedia

Current revision

Crystal structure of the NTF2L domain of human G3BP1 in complex with the USP10 derived peptide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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