1jms

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{{STRUCTURE_1jms| PDB=1jms | SCENE= }}
{{STRUCTURE_1jms| PDB=1jms | SCENE= }}
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'''Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase'''
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===Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase===
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==Overview==
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The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 A resolution reveals a typical DNA polymerase beta-like fold locked in a closed form. In addition, the structures of two different binary complexes, one with an oligonucleotide primer and the other with an incoming ddATP-Co(2+) complex, show that the substrates and the two divalent ions in the catalytic site are positioned in TdT in a manner similar to that described for the human DNA polymerase beta ternary complex, suggesting a common two metal ions mechanism of nucleotidyl transfer in these two proteins. The inability of TdT to accommodate a template strand can be explained by steric hindrance at the catalytic site caused by a long lariat-like loop, which is absent in DNA polymerase beta. However, displacement of this discriminating loop would be sufficient to unmask a number of evolutionarily conserved residues, which could then interact with a template DNA strand. The present structure can be used to model the recently discovered human polymerase mu, with which it shares 43% sequence identity.
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The line below this paragraph, {{ABSTRACT_PUBMED_11823435}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11823435 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11823435}}
==About this Structure==
==About this Structure==
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[[Category: Nucleotidyl transferase]]
[[Category: Nucleotidyl transferase]]
[[Category: Polymerase]]
[[Category: Polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:25:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:28:51 2008''

Revision as of 17:28, 1 July 2008

Image:1jms.png

Template:STRUCTURE 1jms

Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase

Template:ABSTRACT PUBMED 11823435

About this Structure

1JMS is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase., Delarue M, Boule JB, Lescar J, Expert-Bezancon N, Jourdan N, Sukumar N, Rougeon F, Papanicolaou C, EMBO J. 2002 Feb 1;21(3):427-39. PMID:11823435

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