1jqc

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{{STRUCTURE_1jqc| PDB=1jqc | SCENE= }}
{{STRUCTURE_1jqc| PDB=1jqc | SCENE= }}
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'''Mn substituted Ribonucleotide reductase R2 from E. Coli oxidized by hydrogen peroxide and hydroxylamine'''
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===Mn substituted Ribonucleotide reductase R2 from E. Coli oxidized by hydrogen peroxide and hydroxylamine===
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==Overview==
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The di-iron carboxylate proteins constitute a diverse class of non-heme iron enzymes performing a multitude of redox reactions. These reactions usually involve high-valent Fe-oxo species and are thought to be controlled by carboxylate shifts. Owing to their short lifetime, the intermediate structures have so far escaped structural characterization by X-ray crystallography. In an attempt to map the carboxylate conformations available to the protein during different redox states and different ligand environments, we have studied metal-substituted forms of the R2 protein of ribonucleotide reductase from Escherichia coli. In the present work we have solved the crystal structures of Mn-substituted R2 oxidized in two different ways. Oxidation was performed using either nitric oxide or a combination of hydrogen peroxide and hydroxylamine. The two structures are virtually identical, indicating that the oxidation states are the same, most likely a mixed-valent MnII-MnIII centre. One of the carboxylate ligands (D84) adopts a new, so far unseen, conformation, which could participate in the mechanism for radical generation in R2. E238 adopts a bridging-chelating conformation proposed to be important for proper O2 activation but not previously observed in the wild-type enzyme. Probable catalase activity was also observed during the oxidation with H2O2, indicating mechanistic similarities to the di-Mn catalases.
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(as it appears on PubMed at http://www.pubmed.gov), where 11315567 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11315567}}
==About this Structure==
==About this Structure==
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[[Category: Radical protein]]
[[Category: Radical protein]]
[[Category: Ribonucleotide reductase r2]]
[[Category: Ribonucleotide reductase r2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:38:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:38:14 2008''

Revision as of 17:38, 1 July 2008

Image:1jqc.png

Template:STRUCTURE 1jqc

Mn substituted Ribonucleotide reductase R2 from E. Coli oxidized by hydrogen peroxide and hydroxylamine

Template:ABSTRACT PUBMED 11315567

About this Structure

1JQC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of oxidized dinuclear manganese centres in Mn-substituted class I ribonucleotide reductase from Escherichia coli: carboxylate shifts with implications for O2 activation and radical generation., Hogbom M, Andersson ME, Nordlund P, J Biol Inorg Chem. 2001 Mar;6(3):315-23. PMID:11315567

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