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1jv6

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{{STRUCTURE_1jv6| PDB=1jv6 | SCENE= }}
{{STRUCTURE_1jv6| PDB=1jv6 | SCENE= }}
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'''BACTERIORHODOPSIN D85S/F219L DOUBLE MUTANT AT 2.00 ANGSTROM RESOLUTION'''
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===BACTERIORHODOPSIN D85S/F219L DOUBLE MUTANT AT 2.00 ANGSTROM RESOLUTION===
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==Overview==
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Crystal structures are reported for the D85S and D85S/F219L mutants of the light-driven proton/hydroxyl-pump bacteriorhodopsin. These mutants crystallize in the orthorhombic C222(1) spacegroup, and provide the first demonstration that monoolein-based cubic lipid phase crystallization can support the growth of well-diffracting crystals in non-hexagonal spacegroups. Both structures exhibit similar and substantial differences relative to wild-type bacteriorhodopsin, suggesting that they represent inherent features resulting from neutralization of the Schiff base counterion Asp85. We argue that these structures provide a model for the last photocycle intermediate (O) of bacteriorhodopsin, in which Asp85 is protonated, the proton release group is deprotonated, and the retinal has reisomerized to all-trans. Unlike for the M and N photointermediates, where structural changes occur mainly on the cytoplasmic side, here the large-scale changes are confined to the extracellular side. As in the M intermediate, the side-chain of Arg82 is in a downward configuration, and in addition, a pi-cloud hydrogen bond forms between Trp189 NE1 and Trp138. On the cytoplasmic side, there is increased hydration near the surface, suggesting how Asp96 might communicate with the bulk during the rise of the O intermediate.
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{{ABSTRACT_PUBMED_11676543}}
==About this Structure==
==About this Structure==
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[[Category: Ion transport]]
[[Category: Ion transport]]
[[Category: Photoreceptor]]
[[Category: Photoreceptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:57:46 2008''

Revision as of 17:57, 1 July 2008

Image:1jv6.png

Template:STRUCTURE 1jv6

BACTERIORHODOPSIN D85S/F219L DOUBLE MUTANT AT 2.00 ANGSTROM RESOLUTION

Template:ABSTRACT PUBMED 11676543

About this Structure

1JV6 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.

Reference

Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate., Rouhani S, Cartailler JP, Facciotti MT, Walian P, Needleman R, Lanyi JK, Glaeser RM, Luecke H, J Mol Biol. 2001 Oct 26;313(3):615-28. PMID:11676543

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