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1jxb

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{{STRUCTURE_1jxb| PDB=1jxb | SCENE= }}
{{STRUCTURE_1jxb| PDB=1jxb | SCENE= }}
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'''I53A, a point mutant of the cysteine-free variant of E. coli Rnase HI'''
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===I53A, a point mutant of the cysteine-free variant of E. coli Rnase HI===
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==Overview==
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A point mutation (I53A) in the core of Escherichia coli RNase H* is known to destabilize both the native conformation (DeltaG(UN)) and the kinetic intermediate (DeltaG(UI)) by 2 kcal/mole. Here, we have used native-state hydrogen deuterium exchange to ask how this destabilization is propagated throughout the molecule. Stability parameters were obtained for individual residues in I53A and compared with those from the wild-type protein. A destabilization of 2 kcal/mole was observed in residues in the core but was not detected in the periphery of the molecule. These results are consistent with the localized destabilization of the core observed in the early intermediate of the kinetic folding pathway, supporting the resemblance of this kinetic intermediate to the partially unfolded form detected in the native state at equilibrium. A thermodynamic cycle also shows no interaction between Ile 53 and a residue in the periphery. There is, however, an increase in the number of denaturant-independent exchange events in the periphery of I53A, showing that effects of the point mutation are communicated to regions outside the core, although perhaps not through changes in stability. In sum, this work shows that localized regions within a protein can be destabilized independently. Furthermore, it implies a correspondence between the kinetic intermediate and the equilibrium PUF, as the magnitude and localization of the destabilization are the same in both.
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The line below this paragraph, {{ABSTRACT_PUBMED_11847275}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11847275 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11847275}}
==About this Structure==
==About this Structure==
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[[Category: 4-helix bundle]]
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[[Category: Mixed alpha/beta]]
[[Category: Mixed alpha/beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:02:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:00:03 2008''

Revision as of 08:00, 28 July 2008

Image:1jxb.png

Template:STRUCTURE 1jxb

I53A, a point mutant of the cysteine-free variant of E. coli Rnase HI

Template:ABSTRACT PUBMED 11847275

About this Structure

1JXB is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Propagation of a single destabilizing mutation throughout the Escherichia coli ribonuclease HI native state., Spudich G, Lorenz S, Marqusee S, Protein Sci. 2002 Mar;11(3):522-8. PMID:11847275

Page seeded by OCA on Mon Jul 28 11:00:03 2008

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