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1jza

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[[Image:1jza.gif|left|200px]]
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{{STRUCTURE_1jza| PDB=1jza | SCENE= }}
{{STRUCTURE_1jza| PDB=1jza | SCENE= }}
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'''Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing'''
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===Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing===
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==Overview==
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Centruroides sculpturatus Ewing variant 2 toxin (CsE-v2) is a neurotoxin isolated from the venom of a scorpion native to the Arizona desert. The structure of CsE-v2 was solved in two different crystal forms using a combination of molecular replacement and multiple isomorphous replacement techniques. Crystals of CsE-v2 display a temperature-dependent, reversible-phase transition near room temperature. At lower temperature the space group changes from P3(2)21 to P3(1)21 with an approximate doubling of the C-axis. The small-cell structure, which has one molecule per asymmetric unit, has an R factor of 0.229 at 2.8 A resolution. The large-cell structure has two molecules per asymmetric unit and was refined at 2.2 A resolution to an R factor of 0.255. CsE-v2 is a rigid, compact structure with four intrachain disulfide bonds. The structure is similar to other long-chain beta neurotoxins, and the largest differences occur in the last six residues. The high-resolution structure of CsE-v2 corrects an error in the reported C-terminal sequence; the terminal tripeptide sequence is Ser 64-Cys 65-Ser 66 rather than Ser 64-Ser 65-Cys 66. Comparison of CsE-v2 with long-chain alpha toxins reveals four insertions and one deletion, as well as additional residues at the N and C termini. Structural alignment of alpha and beta toxins suggests that the primary distinguishing feature between the two classes is the length of the loop between the second and third strands in a three-strand beta sheet. The shorter loop in alpha toxins exposes a critical lysine side chain, whereas the longer loop in beta toxins buries the corresponding basic residue (either arginine or lysine).
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The line below this paragraph, {{ABSTRACT_PUBMED_11847271}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11847271 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11847271}}
==About this Structure==
==About this Structure==
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[[Category: Noncrystallographic symmetry]]
[[Category: Noncrystallographic symmetry]]
[[Category: Scorpion toxin]]
[[Category: Scorpion toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:06:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:53:24 2008''

Revision as of 20:53, 28 July 2008

Image:1jza.png

Template:STRUCTURE 1jza

Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing

Template:ABSTRACT PUBMED 11847271

About this Structure

1JZA is a Single protein structure of sequence from Centruroides sculpturatus. Full crystallographic information is available from OCA.

Reference

Structure of variant 2 scorpion toxin from Centruroides sculpturatus Ewing., Cook WJ, Zell A, Watt DD, Ealick SE, Protein Sci. 2002 Mar;11(3):479-86. PMID:11847271

Page seeded by OCA on Mon Jul 28 23:53:24 2008

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