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1k02

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{{STRUCTURE_1k02| PDB=1k02 | SCENE= }}
{{STRUCTURE_1k02| PDB=1k02 | SCENE= }}
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'''Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn'''
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===Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn===
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==Overview==
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Glutamine 114 of OYE1 is a well conserved residue in the active site of the Old Yellow Enzyme family. It forms hydrogen bonds to the O2 and N3 of the flavoprotein prosthetic group, FMN. Glutamine 114 was mutated to asparagine, introducing an R-group that is one methylene group shorter. The resultant enzyme was characterized to determine the effect of the mutation on the mechanistic behavior of the enzyme, and the crystal structure was solved to determine the effect of the mutation on the structure of the protein. The Q114N mutation results in little change in the protein structure, moving the amide group of residue 114 out of H-bonding distance, allowing repositioning of the FMN prosthetic group to form new interactions that replace the lost H-bonds. The mutation decreases the ability to bind ligands, as all dissociation constants for substituted phenols are larger than for the wild type enzyme. The rate constant for the reductive half-reaction with beta-NADPH is slightly greater, whereas that for the oxidative half-reaction with 2-cyclohexenone is smaller than for the wild type enzyme. Oxidation with molecular oxygen is biphasic and involves formation and reaction with O(2), a phenomenon that is more pronounced with this mutation than with wild type enzyme. When superoxide dismutase is added to the reaction, we observe a single-phase reaction typical of the wild type enzyme. Turnover reactions using beta-NADPH with 2-cyclohexenone and molecular oxygen were studied to further characterize the mutant enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 11668181 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11668181}}
==About this Structure==
==About this Structure==
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[[Category: Massey, V.]]
[[Category: Massey, V.]]
[[Category: Beta-alpha barrel]]
[[Category: Beta-alpha barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:07:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:48:41 2008''

Revision as of 17:48, 28 July 2008

Image:1k02.png

Template:STRUCTURE 1k02

Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn

Template:ABSTRACT PUBMED 11668181

About this Structure

1K02 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The role of glutamine 114 in old yellow enzyme., Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V, J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:11668181

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