8idq

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'''Unreleased structure'''
 
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The entry 8idq is ON HOLD until Paper Publication
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==Crystal structure of reducing-end xylose-releasing exoxylanase in GH30 from Talaromyces cellulolyticus with xylose==
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<StructureSection load='8idq' size='340' side='right'caption='[[8idq]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8idq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_pinophilus Talaromyces pinophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IDQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8idq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8idq OCA], [https://pdbe.org/8idq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8idq RCSB], [https://www.ebi.ac.uk/pdbsum/8idq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8idq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0B8MZ29_TALPI A0A0B8MZ29_TALPI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TcXyn30A from Talaromyces cellulolyticus, which belongs to subfamily 7 of the glycoside hydrolase family 30 (GH30-7), releases xylose from the reducing end of xylan and xylooligosaccharides (XOSs), the so-called reducing-end xylose-releasing exoxylanase (ReX). In this study, the crystal structures of TcXyn30A with and without xylose at subsite +1 (the binding site of the xylose residue at the reducing end) were determined. This is the first report on the structure of ReX in the family GH30-7. TcXyn30A forms a dimer. The complex structure of TcXyn30A with xylose revealed that subsite +1 is located at the dimer interface. TcXyn30A recognizes xylose at subsite +1 composed of amino acid residues from each monomer and blocks substrate binding to subsite +2 by dimer formation. Thus, the dimeric conformation is responsible for ReX activity. The structural comparison between TcXyn30A and the homologous enzyme indicated that subsite -2 is composed of assembled three stacked Trp residues, Trp49, Trp333, and Trp334, allowing TcXyn30A to accommodate xylan and any branched XOSs decorated with a substitution such as alpha-1,2-linked 4-O-methyl-d-glucuronic acid or alpha-1,2- and/or -1,3-linked L-arabinofuranose. These findings provide an insight into the structural determinants for ReX activity of TcXyn30A.
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Authors: Nakamichi, Y., Watanabe, M., Fujii, T., Inoue, H., Morita, T.
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Crystal structure of reducing-end xylose-releasing exoxylanase in subfamily 7 of glycoside hydrolase family 30.,Nakamichi Y, Watanabe M, Fujii T, Inoue H, Morita T Proteins. 2023 May 5. doi: 10.1002/prot.26505. PMID:37144255<ref>PMID:37144255</ref>
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Description: Crystal structure of reducing-end xylose-releasing exoxylanase in GH30 from Talaromyces cellulolyticus with xylose
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Nakamichi, Y]]
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<div class="pdbe-citations 8idq" style="background-color:#fffaf0;"></div>
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[[Category: Watanabe, M]]
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== References ==
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[[Category: Morita, T]]
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<references/>
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[[Category: Fujii, T]]
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__TOC__
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[[Category: Inoue, H]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Talaromyces pinophilus]]
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[[Category: Fujii T]]
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[[Category: Inoue H]]
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[[Category: Morita T]]
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[[Category: Nakamichi Y]]
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[[Category: Watanabe M]]

Revision as of 07:03, 18 May 2023

Crystal structure of reducing-end xylose-releasing exoxylanase in GH30 from Talaromyces cellulolyticus with xylose

PDB ID 8idq

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