4rl5

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the Arabidopsis exocyst subunit exo70 family protein A1

Structural highlights

4rl5 is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

E70A1_ARATH Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane during regulated or polarized secretion. Involved in polarized cell growth and organ morphogenesis. Involved in polarized cell growth and organ morphogenesis. During cytokinesis, involved in cell plate initiation, cell plate maturation and formation of new primary cell wall. Participates in polarized pectin delivery required for the polarized development of the mucilage-producing volcano cells of the seed coat. Involved in the recycling and localization of auxin efflux carriers PIN1 and PIN2, and thus in polar auxin transport regulation. Functions in vesicle trafficking in tracheary elements to regulate patterned secondary cell wall thickening.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The exocyst complex regulates the last steps of exocytosis, which is essential to organisms across kingdoms. In humans, its dysfunction is correlated with several significant diseases, such as diabetes and cancer progression. Investigation of the dynamic regulation of the evolutionarily conserved exocyst-related processes using mutants in genetically tractable organisms such as Arabidopsis thaliana is limited by the lethality or the severity of phenotypes. We discovered that the small molecule Endosidin2 (ES2) binds to the EXO70 (exocyst component of 70 kDa) subunit of the exocyst complex, resulting in inhibition of exocytosis and endosomal recycling in both plant and human cells and enhancement of plant vacuolar trafficking. An EXO70 protein with a C-terminal truncation results in dominant ES2 resistance, uncovering possible distinct regulatory roles for the N terminus of the protein. This study not only provides a valuable tool in studying exocytosis regulation but also offers a potentially new target for drugs aimed at addressing human disease.

Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis.,Zhang C, Brown MQ, van de Ven W, Zhang ZM, Wu B, Young MC, Synek L, Borchardt D, Harrison R, Pan S, Luo N, Huang YM, Ghang YJ, Ung N, Li R, Isley J, Morikis D, Song J, Guo W, Hooley RJ, Chang CA, Yang Z, Zarsky V, Muday GK, Hicks GR, Raikhel NV Proc Natl Acad Sci U S A. 2015 Nov 25. pii: 201521248. PMID:26607451^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Synek L, Schlager N, Elias M, Quentin M, Hauser MT, Zarsky V. AtEXO70A1, a member of a family of putative exocyst subunits specifically expanded in land plants, is important for polar growth and plant development. Plant J. 2006 Oct;48(1):54-72. Epub 2006 Aug 31. PMID:16942608 doi:http://dx.doi.org/10.1111/j.1365-313X.2006.02854.x
↑ Kulich I, Cole R, Drdova E, Cvrckova F, Soukup A, Fowler J, Zarsky V. Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1 are involved in the localized deposition of seed coat pectin. New Phytol. 2010 Oct;188(2):615-25. doi: 10.1111/j.1469-8137.2010.03372.x. Epub, 2010 Jul 2. PMID:20618910 doi:http://dx.doi.org/10.1111/j.1469-8137.2010.03372.x
↑ Fendrych M, Synek L, Pecenkova T, Toupalova H, Cole R, Drdova E, Nebesarova J, Sedinova M, Hala M, Fowler JE, Zarsky V. The Arabidopsis exocyst complex is involved in cytokinesis and cell plate maturation. Plant Cell. 2010 Sep;22(9):3053-65. doi: 10.1105/tpc.110.074351. Epub 2010 Sep, 24. PMID:20870962 doi:http://dx.doi.org/10.1105/tpc.110.074351
↑ Drdova EJ, Synek L, Pecenkova T, Hala M, Kulich I, Fowler JE, Murphy AS, Zarsky V. The exocyst complex contributes to PIN auxin efflux carrier recycling and polar auxin transport in Arabidopsis. Plant J. 2013 Mar;73(5):709-19. doi: 10.1111/tpj.12074. Epub 2013 Jan 10. PMID:23163883 doi:http://dx.doi.org/10.1111/tpj.12074
↑ Li S, Chen M, Yu D, Ren S, Sun S, Liu L, Ketelaar T, Emons AM, Liu CM. EXO70A1-mediated vesicle trafficking is critical for tracheary element development in Arabidopsis. Plant Cell. 2013 May;25(5):1774-86. doi: 10.1105/tpc.113.112144. Epub 2013 May, 24. PMID:23709627 doi:http://dx.doi.org/10.1105/tpc.113.112144
↑ Zhang C, Brown MQ, van de Ven W, Zhang ZM, Wu B, Young MC, Synek L, Borchardt D, Harrison R, Pan S, Luo N, Huang YM, Ghang YJ, Ung N, Li R, Isley J, Morikis D, Song J, Guo W, Hooley RJ, Chang CA, Yang Z, Zarsky V, Muday GK, Hicks GR, Raikhel NV. Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis. Proc Natl Acad Sci U S A. 2015 Nov 25. pii: 201521248. PMID:26607451 doi:http://dx.doi.org/10.1073/pnas.1521248112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4rl5"

Categories: Arabidopsis thaliana | Large Structures | Song J | Zhang C | Zhang Z-M

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4rl5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RL5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rl5 OCA], [https://pdbe.org/4rl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rl5 RCSB], [https://www.ebi.ac.uk/pdbsum/4rl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rl5 ProSAT]</span></td></tr>
 </table>

4rl5

From Proteopedia

Current revision

Crystal structure of the Arabidopsis exocyst subunit exo70 family protein A1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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