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2n32

From Proteopedia

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Current revision

NMR solution structure of the N-terminal domain of NisI, a lipoprotein from Lactococcus lactis which confers immunity against nisin

Structural highlights

2n32 is a 1 chain structure with sequence from Lactococcus lactis subsp. lactis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NISI_LACLL Involved in immunity against exogenously supplied nisin.

Publication Abstract from PubMed

Many Gram-positive bacteria produce lantibiotics, genetically encoded and posttranslationally modified peptide antibiotics, which inhibit the growth of other Gram-positive bacteria. To protect themselves against their own lantibiotics these bacteria express a variety of immunity proteins including the LanI lipoproteins. The structural and mechanistic basis for LanI-mediated lantibiotic immunity is not yet understood. Lactococcus lactis produces the lantibiotic nisin, which is widely used as a food preservative. Its LanI protein NisI provides immunity against nisin but not against structurally very similar lantibiotics from other species such as subtilin from Bacillus subtilis. To understand the structural basis for LanI-mediated immunity and their specificity we investigated the structure of NisI. We found that NisI is a two-domain protein. Surprisingly, each of the two NisI domains has the same structure as the LanI protein from B. subtilis, SpaI, despite the lack of significant sequence homology. The two NisI domains and SpaI differ strongly in their surface properties and function. Additionally, SpaI-mediated lantibiotic immunity depends on the presence of a basic unstructured N-terminal region that tethers SpaI to the membrane. Such a region is absent from NisI. Instead, the N-terminal domain of NisI interacts with membranes but not with nisin. In contrast, the C-terminal domain specifically binds nisin and modulates the membrane affinity of the N-terminal domain. Thus, our results reveal an unexpected structural relationship between NisI and SpaI and shed light on the structural basis for LanI mediated lantibiotic immunity.

The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin.,Hacker C, Christ NA, Duchardt-Ferner E, Korn S, Gobl C, Berninger L, Dusterhus S, Hellmich UA, Madl T, Kotter P, Entian KD, Wohnert J J Biol Chem. 2015 Nov 27;290(48):28869-86. doi: 10.1074/jbc.M115.679969. Epub, 2015 Oct 12. PMID:26459561^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hacker C, Christ NA, Duchardt-Ferner E, Korn S, Gobl C, Berninger L, Dusterhus S, Hellmich UA, Madl T, Kotter P, Entian KD, Wohnert J. The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin. J Biol Chem. 2015 Nov 27;290(48):28869-86. doi: 10.1074/jbc.M115.679969. Epub, 2015 Oct 12. PMID:26459561 doi:http://dx.doi.org/10.1074/jbc.M115.679969

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n32"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2n32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N32 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n32 OCA], [https://pdbe.org/2n32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n32 RCSB], [https://www.ebi.ac.uk/pdbsum/2n32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n32 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n32 OCA], [https://pdbe.org/2n32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n32 RCSB], [https://www.ebi.ac.uk/pdbsum/2n32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n32 ProSAT]</span></td></tr>
 </table>
 == Function ==

2n32

From Proteopedia

Current revision

NMR solution structure of the N-terminal domain of NisI, a lipoprotein from Lactococcus lactis which confers immunity against nisin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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