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1j3y
From Proteopedia
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| - | [[Image:1j3y. | + | [[Image:1j3y.jpg|left|200px]]<br /><applet load="1j3y" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1j3y" size=" | + | |
caption="1j3y, resolution 1.55Å" /> | caption="1j3y, resolution 1.55Å" /> | ||
'''Direct observation of photolysis-induced tertiary structural changes in human hemoglobin; Crystal structure of alpha(Fe)-beta(Ni) hemoglobin (laser photolysed)'''<br /> | '''Direct observation of photolysis-induced tertiary structural changes in human hemoglobin; Crystal structure of alpha(Fe)-beta(Ni) hemoglobin (laser photolysed)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1J3Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HEM, CMO, HNI and 2FU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1J3Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=CMO:'>CMO</scene>, <scene name='pdbligand=HNI:'>HNI</scene> and <scene name='pdbligand=2FU:'>2FU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tertiary structure changes]] | [[Category: tertiary structure changes]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:05:04 2008'' |
Revision as of 14:05, 15 February 2008
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Direct observation of photolysis-induced tertiary structural changes in human hemoglobin; Crystal structure of alpha(Fe)-beta(Ni) hemoglobin (laser photolysed)
Contents |
Overview
Human Hb, an alpha2beta2 tetrameric oxygen transport protein that switches, from a T (tense) to an R (relaxed) quaternary structure during, oxygenation, has long served as a model for studying protein allostery in, general. Time-resolved spectroscopic measurements after photodissociation, of CO-liganded Hb have played a central role in exploring both protein, dynamical responses and molecular cooperativity, but the direct, visualization and the structural consequences of photodeligation have not, yet been reported. Here we present an x-ray study of structural changes, induced by photodissociation of half-liganded T-state and fully liganded, R-state human Hb at cryogenic temperatures (25-35 K). On photodissociation, of CO, structural changes involving the heme and the F-helix are more, marked in the alpha subunit than in the beta subunit, and more subtle in, the R state than in the T state. Photodeligation causes a significant, sliding motion of the T-state beta heme. Our results establish that the, structural basis of the low affinity of the T state is radically different, between the subunits, because of differences in the packing and chemical, tension at the hemes.
Disease
Known diseases associated with this structure: Erythremias, alpha- OMIM:[141800], Erythremias, beta- OMIM:[141900], Erythrocytosis OMIM:[141850], HPFH, deletion type OMIM:[141900], Heinz body anemia OMIM:[141850], Heinz body anemias, alpha- OMIM:[141800], Heinz body anemias, beta- OMIM:[141900], Hemoglobin H disease OMIM:[141850], Hypochromic microcytic anemia OMIM:[141850], Methemoglobinemias, alpha- OMIM:[141800], Methemoglobinemias, beta- OMIM:[141900], Sickle cell anemia OMIM:[141900], Thalassemia, alpha- OMIM:[141850], Thalassemia-beta, dominant inclusion-body OMIM:[141900], Thalassemias, alpha- OMIM:[141800], Thalassemias, beta- OMIM:[141900]
About this Structure
1J3Y is a Protein complex structure of sequences from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Direct observation of photolysis-induced tertiary structural changes in hemoglobin., Adachi S, Park SY, Tame JR, Shiro Y, Shibayama N, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7039-44. Epub 2003 May 28. PMID:12773618
Page seeded by OCA on Fri Feb 15 16:05:04 2008
Categories: Homo sapiens | Protein complex | Adachi, S. | Park, S.Y. | RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative. | Shibayama, N. | Shiro, Y. | Tame, J.R.H. | 2FU | CMO | HEM | HNI | Crystal photolysis | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Tertiary structure changes
