2n5z

From Proteopedia

(Difference between revisions)

Current revision

Mycobacterium tuberculosis: a dynamic view of the resuscitation promoting factor RpfC catalytic domain

Structural highlights

2n5z is a 1 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPFC_MYCTU Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity.^[1] Stimulates growth of stationary phase M.bovis (a slow-growing Mycobacterium), reduces the lag phase of diluted fast-growers M.smegmatis and Micrococcus luteus. Sequential gene disruption indicates RpfB and RpfE are higher than RpfD and RpfC in functional hierarchy.^[2]

Publication Abstract from PubMed

Mycobacterium tuberculosis latent infection is maintained for years with no clinical symptoms and no adverse effects for the host. The mechanism through which dormant M. tuberculosis resuscitates and enters the cell cycle leading to tuberculosis is attracting much interest. The RPF family of proteins has been found to be responsible for bacteria resuscitation and normal proliferation. This family of proteins in M. tuberculosis is composed by five homologues (named RpfA-E) and understanding their conformational, structural and functional peculiarities is crucial to the design of therapeutic strategies.Therefore, we report the structural and dynamics characterization of the catalytic domain of RpfC from M. tubercolosis by combining Nuclear Magnetic Resonance, Circular Dichroism and Molecular Dynamics data. We also show how the formation of a disulfide bridge, highly conserved among the homologues, is likely to modulate the shape of the RpfC hydrophobic catalytic cleft. This might result in a protein function regulation via a "conformational editing" through a disulfide bond formation.

NMR Structure and Dynamics of the Resuscitation Promoting Factor RpfC Catalytic Domain.,Maione V, Ruggiero A, Russo L, De Simone A, Pedone PV, Malgieri G, Berisio R, Isernia C PLoS One. 2015 Nov 17;10(11):e0142807. doi: 10.1371/journal.pone.0142807., eCollection 2015. PMID:26576056^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mukamolova GV, Turapov OA, Young DI, Kaprelyants AS, Kell DB, Young M. A family of autocrine growth factors in Mycobacterium tuberculosis. Mol Microbiol. 2002 Nov;46(3):623-35. PMID:12410821
↑ Mukamolova GV, Turapov OA, Young DI, Kaprelyants AS, Kell DB, Young M. A family of autocrine growth factors in Mycobacterium tuberculosis. Mol Microbiol. 2002 Nov;46(3):623-35. PMID:12410821
↑ Maione V, Ruggiero A, Russo L, De Simone A, Pedone PV, Malgieri G, Berisio R, Isernia C. NMR Structure and Dynamics of the Resuscitation Promoting Factor RpfC Catalytic Domain. PLoS One. 2015 Nov 17;10(11):e0142807. doi: 10.1371/journal.pone.0142807., eCollection 2015. PMID:26576056 doi:http://dx.doi.org/10.1371/journal.pone.0142807

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n5z"

Categories: Large Structures | Mycobacterium tuberculosis H37Rv | Isernia C | Maione V | Russo L

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2n5z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N5Z FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5z OCA], [https://pdbe.org/2n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n5z RCSB], [https://www.ebi.ac.uk/pdbsum/2n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n5z ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5z OCA], [https://pdbe.org/2n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n5z RCSB], [https://www.ebi.ac.uk/pdbsum/2n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n5z ProSAT]</span></td></tr>
 </table>
 == Function ==

2n5z

From Proteopedia

Current revision

Mycobacterium tuberculosis: a dynamic view of the resuscitation promoting factor RpfC catalytic domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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