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Sandbox Reserved 1777
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== Binding Pocket == | == Binding Pocket == | ||
| - | Once all the domains are bound NTpS binds to PP1C in the [https://en.wikipedia.org/wiki/Active_site active site]. Once the NTpS is bound it becomes dephosphorylated and the complex falls apart. NTpS is dephosphorylated to prevent the active dimeric [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311149/#:~:text=Full%20activation%20of%20Raf%20requires,plasma%20membrane%20(Roy%20et%20al. RAF] from inactivating and changing into its monomeric structure. The NTpS active site is surrounded by hydrophobic and acidic regions. These regions are located on the surface of PP1C whereas the active site is placed further into the structure. It is thought that these regions help the ligand bind to the active site by making interactions that will lead NTpS into the protein. There is still some uncertainty as to how the substrate selectivity works but these regions could play an essential role in it. Specifically, the C-terminal of the pS from NTpS would bind to the hydrophobic region on PP1C. | + | |
| + | Once all the domains are bound NTpS binds to PP1C in the [https://en.wikipedia.org/wiki/Active_site active site]. Once the NTpS is bound it becomes dephosphorylated and the complex falls apart. NTpS is dephosphorylated to prevent the active dimeric [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311149/#:~:text=Full%20activation%20of%20Raf%20requires,plasma%20membrane%20(Roy%20et%20al. RAF] from inactivating and changing into its monomeric structure. The NTpS active site is surrounded by hydrophobic and acidic regions along with C-terminal. (<scene name='95/952705/Pp1c_active_site/3'>PP1C Active Site</scene>). These regions are located on the surface of PP1C whereas the active site is placed further into the structure. It is thought that these regions help the ligand bind to the active site by making interactions that will lead NTpS into the protein. There is still some uncertainty as to how the substrate selectivity works but these regions could play an essential role in it. Specifically, the C-terminal of the pS from NTpS would bind to the hydrophobic region on PP1C. | ||
NTpS can bind to the PP1C active site without PP1C also being bound to SHOC2 and MRAS, however the catalytic activity is much slower and the reaction is less efficient. Also for this event to occur NTpS would need to be exposed from its binding site in the inactive RAF complex. A RAS has to bind to RAF to expose the NTpS allowing PP1C and NTpS to bind. | NTpS can bind to the PP1C active site without PP1C also being bound to SHOC2 and MRAS, however the catalytic activity is much slower and the reaction is less efficient. Also for this event to occur NTpS would need to be exposed from its binding site in the inactive RAF complex. A RAS has to bind to RAF to expose the NTpS allowing PP1C and NTpS to bind. | ||
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== Relevance == | == Relevance == | ||
| - | <scene name='95/952705/Pp1c_active_site/1'>TextToBeDisplayed</scene> | ||
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| - | <scene name='95/952705/Pp1c_active_site/2'>PP1C Active Site</scene> | ||
| - | <scene name='95/952705/Pp1c_active_site/3'>TextToBeDisplayed</scene> | ||
== Structural highlights == | == Structural highlights == | ||
Revision as of 21:55, 22 March 2023
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References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
