1kay

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{{STRUCTURE_1kay| PDB=1kay | SCENE= }}
{{STRUCTURE_1kay| PDB=1kay | SCENE= }}
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'''70KD HEAT SHOCK COGNATE PROTEIN ATPASE DOMAIN, K71A MUTANT'''
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===70KD HEAT SHOCK COGNATE PROTEIN ATPASE DOMAIN, K71A MUTANT===
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==Overview==
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It has been proposed that lysine 71 of the bovine 70-kDa heat shock cognate protein might participate in catalysis of ATP hydrolysis by stabilizing an H2O molecule or an OH- ion for nucleophilic attack on the gamma-phosphate of the nucleotide (Flaherty, K. M., Wilbanks, S. M., DeLuca-Flaherty, C., and McKay, D. B. (1994) J. Biol. Chem. 12899-12907; Wilbanks, S. M., DeLuca-Flaherty, C., and McKay, D. B. (1994) J. Biol. Chem. 269, 12893-12898). To test this hypothesis, lysine 71 of the ATPase fragment 70-kDa heat shock cognate protein has been mutated to glutamic acid, methionine, and alanine; and the kinetic and structural properties of the mutant proteins have been determined. All three mutant proteins are devoid of measurable ATP hydrolysis activity. Crystal structures of the mutant proteins have been determined to a resolution of 1.7 A; all three have ATP in the nucleotide binding site. These data identify lysine 71 as a residue that is essential for chemical hydrolysis of ATP.
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(as it appears on PubMed at http://www.pubmed.gov), where 8663302 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8663302}}
==About this Structure==
==About this Structure==
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[[Category: Heat shock]]
[[Category: Heat shock]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:31:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:03:29 2008''

Revision as of 07:03, 2 July 2008

Image:1kay.png

Template:STRUCTURE 1kay

70KD HEAT SHOCK COGNATE PROTEIN ATPASE DOMAIN, K71A MUTANT

Template:ABSTRACT PUBMED 8663302

About this Structure

1KAY is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Lysine 71 of the chaperone protein Hsc70 Is essential for ATP hydrolysis., O'Brien MC, Flaherty KM, McKay DB, J Biol Chem. 1996 Jul 5;271(27):15874-8. PMID:8663302

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