1kcn

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{{STRUCTURE_1kcn| PDB=1kcn | SCENE= }}
{{STRUCTURE_1kcn| PDB=1kcn | SCENE= }}
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'''Structure of e109 Zeta Peptide, an Antagonist of the High-Affinity IgE Receptor'''
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===Structure of e109 Zeta Peptide, an Antagonist of the High-Affinity IgE Receptor===
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==Overview==
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Recently we described a family of peptides, unrelated in sequence to IgE, that form stable beta-hairpins in solution and inhibit IgE activity in the microM range [Nakamura, G. R., Starovasnik, M. A., Reynolds, M. E. &amp; Lowman, H. B. (2001) Biochemistry 40, 9828-9835]. Using an expanded set of peptide-phage libraries, we found a simpler motif, X(2)CPX(2)CYX, for binding to the high-affinity IgE receptor. In solution, one of these peptides spontaneously formed a covalent antiparallel dimer. We subsequently linked these monomers in a single-chain construct on phage and optimized receptor binding. Ultimately, peptides with 30 nM affinity were produced. NMR studies showed that the peptide adopts a stable fold consisting of two "zeta" (zeta)-shaped moieties. Structure-activity analyses reveal a single binding site created by the zeta-dimer, with two tyrosine residues important for structural stability and two proline residues important for Fc epsilon RI binding. The peptides inhibit histamine release from cultured cells and are extremely stable in biological fluids. The zeta peptides appear to act as competitive IgE inhibitors and suggest possibilities for design of novel IgE antagonists.
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(as it appears on PubMed at http://www.pubmed.gov), where 11830661 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11830661}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCN OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCN OCA].
==Reference==
==Reference==
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[[Category: Disulfide-bonded]]
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[[Category: Helical]]
[[Category: Helical]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:34:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:08:06 2008''

Revision as of 07:08, 2 July 2008

Image:1kcn.png

Template:STRUCTURE 1kcn

Structure of e109 Zeta Peptide, an Antagonist of the High-Affinity IgE Receptor

Template:ABSTRACT PUBMED 11830661

About this Structure

Full experimental information is available from OCA.

Reference

Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor., Nakamura GR, Reynolds ME, Chen YM, Starovasnik MA, Lowman HB, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1303-8. PMID:11830661

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