BASIL2023GV1ZBS

1ZBS is a protein with unknown function discovered through a genomics project of predictive folding. The goal of this project was to determine what the function of protein 1ZBS was using a combination of computational analysis and physical lab work.

For this project, we started by doing our computational work, which includes BLAST, DALI, SPRITE, Net-GO, and the Docking analysis, while we started transforming our bacteria, and over-expressing and purifying our protein for the kinase assays.

Sequence Alignments

BLAST-P

The first type of analysis that was done was using Protein Blast from NCBI. The analysis was first done as it only gave very broad information about the potential function of the protein, by matching domains across other proteins with known functions.

Structural Alignments

This is our DALI alignment of 1ZBS and 2CH5. Image:Https://proteopedia.org/wiki/images/4/48/DALI alignment.png

Substrate Possibilities

Kinase Assays

Conclusion

This is a sample scene created with SAT to </scene> by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.